{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:30:23Z","timestamp":1791819023650,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T00:00:00Z","timestamp":1746230400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Human parvovirus B19 (B19V) is responsible for a wide clinical spectrum ranging from asymptomatic infection, through mild disease, up to life\u2010threatening one. Outbreaks are registered every 3\u20134 years, and a recent international alert for a new outbreak has been released. The experience of B19 virus circulation in a 600\u2010bed tertiary care pediatric hospital in Rome from 2018 to 2024 is reported here. This retrospective study involved a total of 9695 blood samples (about 8500 patients), 11 amniotic fluids (11 pregnant women), and 10\u2009827 sera (about 9500 patients), processed in the Virology Unit of Bambino Gesu\u0300 Children's Hospital in Rome for B19V direct and indirect detection. In our population, the annual positivity rate for B19V DNA ranged from 0.8% in 2023 to 9.8% in 2018 and 32.8% in 2024; the same trend resulted from the analysis of the immunoglobulins M and G anti\u2010B19V. Focusing on the last year, 314 patients resulted positive for B19V DNA detection: 204\/314 (65%) had a primary infection, 150\/204 (73.5%) were hospitalized, and 17\/150 (11.3%) needed Intensive Care Unit (ICU) for cardiovascular, central nervous, and gastrointestinal pathologies. Two patients died from myocarditis. Among patients with the most severe clinical picture, over half had no concurring disease, and one patient died. Four amniotic fluids were positive for pregnant women who came to our observation. B19V typing of a subset of samples revealed the presence of only subtype 1A and a low intragenotypic diversity between strains from severe and mild disease. In conclusion, in 2024, a significant increase in B19V circulation was observed with profound effects on clinical outcome and consequent hospitalisation, either in patients with comorbidities or those without. This widespread circulation of the virus also had an impact on infections in pregnancy, with the known severe consequences for unborn children.<\/jats:p>","DOI":"10.1002\/jmv.70380","type":"journal-article","created":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T07:20:02Z","timestamp":1746256802000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Parvovirus B19 Rebound"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9578-7429","authenticated-orcid":false,"given":"Stefania","family":"Ranno","sequence":"first","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Russo","sequence":"additional","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luna","family":"Colagrossi","sequence":"additional","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Fox","sequence":"additional","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Velia Chiara","family":"Di Maio","sequence":"additional","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Linardos","sequence":"additional","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonarda","family":"Gentile","sequence":"additional","affiliation":[{"name":"Microbiology and Diagnostic Immunology Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaria","family":"Marotta","sequence":"additional","affiliation":[{"name":"Pediatric Emergency Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Cristaldi","sequence":"additional","affiliation":[{"name":"Pediatric Emergency Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Campana","sequence":"additional","affiliation":[{"name":"Pediatrics Unit, Bambino Ges\u00f9 Children's Hospital, IRCCS Rome 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