{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T01:56:20Z","timestamp":1755222980889,"version":"3.43.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Aimed to comprehensively investigate the presence of neural autoantibodies in the cerebrospinal fluid (CSF) and plasma of COVID\u201019 patients experiencing neurological complications during the Omicron wave in China. Forty consecutive COVID\u201019 patients with severe neurological complications and 15 disease controls (DC) were enrolled. Neural autoantibodies were detected using both the indirect immunofluorescence assay (IFA) on mouse brain tissue and the Brain\u2010neuronal\u2010antigen microarray. Our results indicated a significantly higher prevalence of neural autoantibodies in the CSF (62.16% vs. 0.0%) and plasma (38.71% vs. 13.33%) of COVID\u201019 patients compared to DC. Additionally, we identified 12 upregulated intrathecal IgG autoantibodies with differential levels between COVID\u201019 patients and DC, as well as 51 upregulated IgG autoantibodies in plasma. A high prevalence of anti\u2010mGluR2 antibodies (13.33%) in COVID\u201019 patients was confirmed by cell\u2010based assays. Western blot analysis showed these antibodies cross\u2010react with both the nucleocapsid (N) and spike (S) proteins of SARS\u2010CoV\u20102. Notably, strong binding to both the S protein's RBD\u2010Fc and mGluR2 was observed, an association that was substantiated by bioinformatics analysis evaluating the similarity between SARS\u2010CoV\u20102 proteins and the targeted antigens on the microarray. This finding hints at a potential cross\u2010reactivity between anti\u2010mGluR2 antibodies and the S protein in COVID\u201019 patients.<\/jats:p>","DOI":"10.1002\/jmv.70381","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T10:26:37Z","timestamp":1746699997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identifying Neurological Autoantibodies in COVID\u201019: mGluR2 as a Marker of Immune Dysregulation During the Omicron Outbreak in China"],"prefix":"10.1002","volume":"97","author":[{"given":"Ziyan","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Clinical laboratory, Peking Union Medical College Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Neurology, 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