{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T01:56:21Z","timestamp":1755222981691,"version":"3.43.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Memory B cells (MBCs) are responsible for maintaining long\u2010lasting functional B\u2010cell immune responses. Little is known about the kinetics of peripheral blood (PB) SARS\u2010CoV\u20102 vaccine\u2010induced MBCs in end\u2010stage chronic kidney disease (CKD) patients undergoing replacement therapies. We investigated this issue in this prospective, observational cohort study including 27 patients (9 females and 18 males; median age, 68.4 years, range 48\u201382) comprising 20 hemodialysis patients and 7 Kidney transplant recipients. SARS\u2010CoV\u20102\u2010Receptor\u2010Binding Domain (RBD)\u2010targeted PB\u2010MBCs were enumerated by flow cytometry using a tetramer\u2010binding assay after the second COVID\u201019 mRNA vaccine dose (Post\u20102D), before (Pre\u20103D), and after the first mRNA vaccine booster dose (Post\u20103D). Commercially available electrochemiluminescent immunoassays were used to measure total anti\u2010RBD antibodies targeting an IgG against the S trimeric protein. Overall, 18\/27 patients (66.6%) exhibited detectable RBD\u2010MBC responses at Post\u20102D, 12\/27 (44.4%) at Pre\u20103D, and 16\/27 (59.2%) at Post\u20103D. RBD\u2010MBC levels dropped non\u2010significantly between post\u20102D and Pre\u20103D (<jats:italic>p<\/jats:italic>\u2009=\u20090.38). A nonsignificant increase in RBD\u2010MBCs was noticed post\u20103D (<jats:italic>p<\/jats:italic>\u2009=\u20090.65). Overall, both antibody specificities displayed the same dynamics but the drop in anti\u2010trimeric spike antibody levels between Post\u20102D and Pre\u20103D and increases post\u20103D were statistically significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). No correlation (rho\u2009=\u20090.05; <jats:italic>p<\/jats:italic>\u2009=\u20090.64) was observed between total antibodies against RBD and RBD\u2010MBC counts. The correlation between IgG antibodies against the trimeric S protein and SARS\u2010CoV\u20102 RBD\u2010MBC counts was very weak (rho, 0.18; <jats:italic>p<\/jats:italic>\u2009=\u20090.11). In summary, waning RBD\u2010MBC counts Pre\u20103D and increases post\u20103D are less marked than that of anti\u2010RBD and anti\u2010S trimeric antibodies.<\/jats:p>","DOI":"10.1002\/jmv.70382","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T10:46:42Z","timestamp":1746528402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamics of SARS\u2010CoV\u20102 Spike Receptor\u2010Binding Domain\u2010Targeted Specific Peripheral Memory B Cells in Patients With End\u2010Stage Chronic Kidney Disease Undergoing Replacement Therapy Following COVID\u201019 Vaccination"],"prefix":"10.1002","volume":"97","author":[{"given":"\u00c1ngela","family":"S\u00e1nchez\u2010Simarro","sequence":"first","affiliation":[{"name":"Microbiology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nayara","family":"Panizo","sequence":"additional","affiliation":[{"name":"Nephrology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estela","family":"Gim\u00e9nez","sequence":"additional","affiliation":[{"name":"Microbiology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"},{"name":"Nephrology Service, Transplant Unit Hospital Universitario Dr. Peset Valencia Spain"},{"name":"CIBER de Enfermedades Infecciosas Instituto de Salud Carlos III Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliseo","family":"Albert","sequence":"additional","affiliation":[{"name":"Microbiology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Montomoli","sequence":"additional","affiliation":[{"name":"Nephrology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Sanchis","sequence":"additional","affiliation":[{"name":"Nephrology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Kanter","sequence":"additional","affiliation":[{"name":"Nephrology Service, Transplant Unit Hospital Universitario Dr. Peset Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"G\u00f3rriz","sequence":"additional","affiliation":[{"name":"Nephrology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"},{"name":"Department of Medicine School of Medicine, University of Valencia Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-4110","authenticated-orcid":false,"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Microbiology Service Hospital Cl\u00ednico Universitario de Valencia, INCLIVA Health Research Institute Valencia Spain"},{"name":"CIBER de Enfermedades Infecciosas Instituto de Salud Carlos III Madrid Spain"},{"name":"Department of Microbiology School of Medicine, University of Valencia Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2521-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2020.07.030"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00570-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-99713-y"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.akdh.2024.03.004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/nep.13741"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.827859"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000536308"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad162"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14081822"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00617-5"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13091"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01248-5"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.13372"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00267-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-023-00897-3"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines12101089"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10112275"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.825813"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1445653"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000005119"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1202630"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2024.101458"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1463769"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2021070966"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2023.104833"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70382","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:38:29Z","timestamp":1754905109000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70382"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/jmv.70382"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70382","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70382"}}