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From September 2022 to August 2023, 1150 women aged 25\u201360 were enrolled at the Changzhou Maternal and Child Health Hospital. All participants tested positive for HPV and negative for both cytology and pathology. A follow\u2010up was conducted 6 months later to reassess HPV status and perform colposcopy. BMRT was employed to detect various HPV types and their viral loads. The ROC curve was utilized to determine the viral load cut\u2010off values for different HPV types to predict cervical lesions. From baseline to follow\u2010up, women whose HPV infection cleared were significantly younger than those with persistent HPV infection (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). At baseline, the viral loads of the virus clearance, maintenance, and progression groups demonstrated an increasing trend (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Among women diagnosed with CIN during follow\u2010up, the viral load increased significantly from baseline to follow\u2010up (<jats:italic>p<\/jats:italic>\u2009=\u20090.001). The combination of HPV genotyping and viral load in the initial screening of cervical cancer enhances the prediction and identification of cervical lesions.<\/jats:p>","DOI":"10.1002\/jmv.70386","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T03:12:47Z","timestamp":1748401967000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Human Papillomavirus Genotyping and Viral Load as a Predictor of Cervical Lesions: A Prospective Study"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7176-6802","authenticated-orcid":false,"given":"Yilu","family":"Zhou","sequence":"first","affiliation":[{"name":"Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University Changzhou Key Laboratory of Maternal and Child Health Medicine Changzhou 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