{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T01:56:22Z","timestamp":1755222982680,"version":"3.43.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Bats are natural reservoirs for a diverse range of coronaviruses (CoVs), including those closely related to SARS\u2010CoV and SARS\u2010CoV\u20102, making them crucial for understanding CoV genetics and zoonotic transmission. The exceptional bat diversity in Sarawak, Malaysian Borneo, provides an ideal setting to investigate CoV diversity and potential transmission pathways. This study examined CoV prevalence and diversity in 346 fecal samples from bats across 29 species in northern and western Sarawak, employing two pan\u2010CoV PCR assays: Quan (Q\u2010assay) and Watanabe (W\u2010assay). The Q\u2010assay and W\u2010assay estimated the CoV prevalence to be 14.45% and 12.72%, respectively. The overall true prevalence based on both assays was 22.83%. There was a fair agreement between both assays (<jats:italic>\u03ba<\/jats:italic>\u2009=\u20090.286) with comparable performance in detecting the virus (McNemar <jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). Phylogenetic analyses identified six distinct clades within alphacoronaviruses (\u03b1\u2010CoVs) and betacoronaviruses (\u03b2\u2010CoVs), comprising two unclassified Borneo\u2010Alpha CoVs and four from the subgenera <jats:italic>Minunacovirus<\/jats:italic>, <jats:italic>Rhinacovirus<\/jats:italic>, <jats:italic>Nobecovirus<\/jats:italic>, and <jats:italic>Sarbecovirus<\/jats:italic>. This study represents the first report of Sarawak bat CoVs derived from rectal and fecal samples, addressing a significant knowledge gap. The findings highlight the need for complementary molecular assays to enhance CoV surveillance and deepen understanding of viral ecology in regions of high biodiversity, with implications for zoonotic disease prevention.<\/jats:p>","DOI":"10.1002\/jmv.70389","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T07:48:42Z","timestamp":1747122522000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of Quan and Watanabe Pan\u2010Coronavirus Assays for Bat Coronavirus Diversity in Sarawak, East Malaysia"],"prefix":"10.1002","volume":"97","author":[{"given":"Sultana Parvin","family":"Habeebur\u2010Rahman","sequence":"first","affiliation":[{"name":"Centre for Tropical and Emerging Diseases, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal Ali Anwarali","family":"Khan","sequence":"additional","affiliation":[{"name":"Faculty of Resource Science and Technology Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayasilan","family":"Mohd\u2010Azlan","sequence":"additional","affiliation":[{"name":"Institute of Biodiversity and Environmental Conservation Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melvin","family":"Gumal","sequence":"additional","affiliation":[{"name":"Sarawak Forestry Corporation, Kota Sentosa Sarawak Kuching Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6312-4551","authenticated-orcid":false,"given":"Cheng Siang","family":"Tan","sequence":"additional","affiliation":[{"name":"Centre for Tropical and Emerging Diseases, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Sarawak Emerging Pathogen Surveillance Study Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0394-z"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-015-0422-1"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22975"},{"volume-title":"Photographic Guide to the Bats of Malaysia","year":"2021","author":"Abdullah M. 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