{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:07:34Z","timestamp":1790312854385,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>To evaluate the immediate risk of (pre)cancer for cytology low\u2010grade squamous intraepithelial lesion (LSIL) women infected with or without specific HPV genotype and develop a risk\u2010based management strategy. A total of 4567 LSIL women with extended HPV genotyping and colposcopy results were enrolled according to the inclusive and exclusive criteria. The distribution and immediate cervical intraepithelial neoplasia grade 2 or worse and 3+ or worse (CIN2+\/3+) risks of specific HPV genotypes were assessed using Minimum Estimate, Any Type Estimate, and Hierarchical Attribution Estimate. A risk\u2010based strategy was further established and evaluated. CIN2+\/3+ were 729\/328 cases, including 691\/317 in 3398 HPV\u2010positive and 38\/11 in 1169 HPV\u2010negative women. HPV16, 52, 58, and 18 were the most prevalent genotypes in both HPV\u2010positive and CIN2+\/3+ cases. HPV16, 73, and 33 carried the highest immediate CIN2+\/3+ risk. A risk\u2010based strategy was established, which suggested Group A (HPV 16, 33, 45, 31, 18, 58, 52, 35, 73, 82; with immediate CIN3+ risk of 4.08%\u201322.12%) for immediate colposcopy, Group B (HPV 59, 66, 56, 53) for 6\u2010month follow\u2010up or p16\/Ki\u201067 dual stain or DNA methylation triage, while Group C (HPV 51, 68, 39, 26) for 1\u2010year HPV repeat testing. Compared with conventional strategy, this new strategy showed significantly higher specificity (CIN2+: 52.16% vs. 29.47%, <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u2009409.136, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001; CIN3+: 48.45% vs. 27.32%, <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u2009402.395, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) but similar sensitivity, which could reduce immediate colposcopy referrals by 19.82%. A risk\u2010based triage strategy for LSIL women with extended HPV genotyping could effectively reduce unnecessary colposcopies and maintain high efficacy for CIN2+\/3+ detection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.70404","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T09:39:17Z","timestamp":1747733957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Risk\u2010Based Triage Strategy by Extended HPV Genotyping for Women With LSIL Cytology: A Real\u2010World Study"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1947-569X","authenticated-orcid":false,"given":"Chun","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Medical Centre for Cervical Diseases, Women's Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Department of Gynecology and Obstetrics Xixi Hospital of Hangzhou Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiru","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhe","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Medical Centre for Cervical Diseases, Women's Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Medical Centre for Cervical Diseases, Women's Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5008-9101","authenticated-orcid":false,"given":"Yunfeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Medical Centre for Cervical Diseases, Women's Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Feng","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, Women's Hospital Zhejiang University School of Medicine Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Hangzhou China"},{"name":"Zhejiang Provincial Clinical Research Center for Gynecological Diseases Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,20]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119755"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2020.100293"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00617-5"},{"key":"e_1_2_13_6_1","unstructured":"\u201cIARC Working Group on the Evaluation of Cancer\u2010Preventive Interventions. 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