{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T04:20:29Z","timestamp":1783398029426,"version":"3.54.6"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Human pegivirus (HPgV) is a blood\u2010borne RNA virus belonging to the family <jats:italic>Flaviviridae<\/jats:italic>, and contains two types HPgV\u20101 and HPgV\u20102. HPgV\u20101 infection does not cause acute diseases in healthy individuals but was demonstrated to play beneficial roles in individuals coinfected with HIV\u20101. HPgV\u20101 has a high prevalence in blood donors and the general population worldwide. The long\u2010term consequence of HPgV\u20101 infection in healthy individuals remains unknown. High prevalence of HPgV\u20101 in blood donors raises concerns about the risk of transfusion transmission. Development of rapid and accurate point\u2010of\u2010care testing (POCT) of HPgV\u20101 will facilitate the screening of HPgV\u20101 infection among blood donors. Here, we reported a novel high\u2010fidelity loop\u2010mediated isothermal amplification (HiFi\u2010LAMP) assay for detection of HPgV\u20101 and evaluated its performance in 175 healthy adults from Taizhou, China. The assay exhibits high specificity and sensitivity with limits of detection (LODs) of 122.6\u2013135.7 copies of viral RNA\/25\u2009\u03bcL reaction for various HPgV\u20101 variants and can be completed within 30\u2009min. Clinical validation showed that the assay had a 100% concordance with a previously described RT\u2010qPCR assay for 175 sera from healthy adults, showing 100% sensitivity and specificity. Furthermore, we reported a 28.0% (111\/397) prevalence of HPgV\u20101 in healthy adults in Taizhou, China, with no significant differences between genders and ages. The prevalence is obviously higher than a pooled HPgV\u20101 prevalence of 3.3% in blood donors in China. The novel HPgV\u20101 HiFi\u2010LAMP assay offers a robust, rapid, and cost\u2010effective tool for HPgV\u20101 surveillance to mitigate transfusion risk, especially in resource\u2010limited areas. High prevalence of HPgV\u20101 in healthy adults underscores its potential public health relevance.<\/jats:p>","DOI":"10.1002\/jmv.70423","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T02:59:10Z","timestamp":1748401150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a HiFi\u2010LAMP Assay for Detection of Human Pegivirus Type 1 RNA"],"prefix":"10.1002","volume":"97","author":[{"given":"Xiuli","family":"Zhao","sequence":"first","affiliation":[{"name":"Medical Laboratory of Taizhou Fourth People's Hospital Taizhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuyue","family":"Lu","sequence":"additional","affiliation":[{"name":"China Regional Research Centre International Centre for Genetic Engineering and Biotechnology (ICGEB) Taizhou Jiangsu China"},{"name":"Rohonor Medical Technology (Jiangsu) Co. Ltd.  Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Medical Laboratory of Taizhou Fourth People's Hospital Taizhou Jiangsu China"},{"name":"Shanghai Public Health Clinical Center Fudan University Shanghai China"},{"name":"School of Medicine Anhui University of Science and Technology Huainan Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Qiao","sequence":"additional","affiliation":[{"name":"Medical Laboratory of Taizhou Fourth People's Hospital Taizhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-9857","authenticated-orcid":false,"given":"Chiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Fudan University Shanghai 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