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In this study, we developed a sensitive and efficient platform for pathogen nucleic acid detection by integrating asymmetric nucleic acid sequence\u2010based amplification (asymmetric NASBA), enzyme\u2010DNA molecular complex, and the clustered regularly interspaced short palindromic repeats (CRISPR) system, namely an A\u2010enDMC platform. The target recognition capability of the enzyme\u2010DNA complex operates independently from the signal amplification function of the CRISPR system. By decoupling the CRISPR reaction from the dependence on specific target sequences, the platform's universality and modularity are enhanced. The assay is fast (&lt;\u20091.5 h), highly sensitive (&lt;\u20095\u2009copies\/\u00b5L), and demonstrates no cross\u2010reactivity with other common viruses. Compared to the widely used RT\u2010qPCR method, the platform demonstrates high consistency in detection results, with the detection coincidence rate of 96.77% and a kappa value of 0.87. This platform provides a versatile technological tool for highly sensitive and specific RNA detection, demonstrating its extensive potential in real sample analysis.<\/jats:p>","DOI":"10.1002\/jmv.70426","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T08:48:36Z","timestamp":1748854116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integrating Enzyme\u2010DNA Complex and CRISPR\/Cas12a for Robust Norovirus Detection"],"prefix":"10.1002","volume":"97","author":[{"given":"Junhua","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pediatrics The Affiliated Women and Children's Hospital of Ningbo University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyan","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Affiliated Women and Children's Hospital of Ningbo University Ningbo 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