{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:56Z","timestamp":1786978196898,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Crimean\u2013Congo hemorrhagic fever virus (CCHFV) is a medically important tick\u2010borne virus, causing severe hemorrhagic diseases in humans. There are no approved vaccines and therapeutics for CCHFV infection. The study of CCHFV authentic virus requires biosafety level 3 facilities, hindering the research and development of antivirals. Here we report the generation of a recombinant vesicular stomatitis virus (VSV) bearing both CCHFV glycoprotein precursor (GPC) and EGFP reporter (rVSV\u2010CCHFV\u2010GFP). We also find that the acquisition of an unexpected single R516K mutation in the GPC protein enables the packaging of high\u2010titer pseudotyped particles. The replication\u2010competent rVSV\u2010CCHFV\u2010GFP reporter virus resembles the entry properties of the authentic virus and allows for rapid assessment of susceptible cell lines, neutralizing antibodies, and host entry factors such as heparan sulfate in fluorescence\u2010based assays. This study provides a valuable strategy for packaging of high\u2010titer CCHFV pseudovirus, and the tool generated here can be served for the identification and evaluation of countermeasures against the cell entry of CCHFV.<\/jats:p>","DOI":"10.1002\/jmv.70457","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T04:00:53Z","timestamp":1750392053000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Single Amino Acid Change of R516K Enables Efficient Generation of Vesicular Stomatitis Virus\u2010Based Crimean\u2013Congo Hemorrhagic Fever Reporter Virus"],"prefix":"10.1002","volume":"97","author":[{"given":"Xue","family":"Ma","sequence":"first","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (MOE\/NHC\/CAMS), School of Basic Medical Sciences, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Shanghai Institute of Infectious Disease 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