{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:53:39Z","timestamp":1783356819550,"version":"3.54.6"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study employed single\u2010cell sequencing to analyze immune dynamics in pediatric infectious mononucleosis (IM), focusing on EBV\u2013host interactions. EBV primarily infected B\u2010cell subsets, showing distinct tropism from EBV\u2010associated hemophagocytic syndrome, indicating stage\u2010specific infection patterns. Coexpression of lytic (BALF3\/5) and latent (EBNA\u20103A, LMP\u20102A) genes suggested dynamic infection states. Notably, RPMS1 expression was consistently detected in IM patients, where this oncogenic regulator influences viral latency maintenance and cellular transformation through Notch signaling. NK cell subsets exhibited differential responses: CD16\u2009+\u2009CD56dim NK cells demonstrated enhanced EBV cytotoxicity, while CD16\u2009\u2212\u2009CD56bright NK cells showed immunoregulatory potential. Clonal expansion of IGHV4\u201034\/59 in B cells (involved in EBV gp350 binding and autoimmunity) suggested dual roles in viral neutralization and pathogenesis. Frequent IGHJ4\/IGLJ3 BCR rearrangements informed vaccine design. Elevated COTL1 in T cells correlated with pro\u2010inflammatory cytokine release, indicating therapeutic potential. Dysregulated IRF4\/PRDM1 in plasma cells reflected interindividual EBV control variability. These findings delineate IM immunopathology, linking EBV persistence to clonal expansion and immune evasion, while providing a framework for targeted therapies (NK cell engineering, IGHV4\u2010directed antibodies, COTL1 modulation) to advance EBV\u2010associated disease treatment.<\/jats:p>","DOI":"10.1002\/jmv.70491","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T13:05:52Z","timestamp":1752757552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Unveiling the Immune Landscape: Single\u2010Cell Sequencing of Infectious Mononucleosis Patients"],"prefix":"10.1002","volume":"97","author":[{"given":"Dongshi","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huamin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimei","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Airong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiqian","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanding","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoguang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojiao","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusha","family":"Jiang","sequence":"additional","affiliation":[{"name":"LC\u2010Bio Technologies (Hangzhou) Co. Ltd Hangzhou Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital &amp; Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20161846"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.08.026"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01953-09"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2000.0783"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00754-13"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141553"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.12.001"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-024-01701-0"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00258-20"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.11.041"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601574"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.03027.x"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1001145"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010453"},{"key":"e_1_2_12_16_1","unstructured":"Subspecialty Group of Infectious Diseases the Society of Pediatrics Chinese Medical Association; National Children\u2032s Epstein\u2010Barr Virus Infection Cooperative Group \u201c[Experts Consensus on Diagnosis and Treatment of Epstein\u2010Barr Virus Infection\u2010Related Diseases in Children] \u201dZhonghua Er Ke Za Zhi59 no. 11 (2021): 905\u2013911."},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2019.03.003"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv088"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts714"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baz046"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0694-x"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009834"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28362"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0040027"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301046"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2015.03.014"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006512"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-018-0213-2"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20182031"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085090"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1679"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2019.01.019"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.114696"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3839"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.931630"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.07.016"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08155"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085422"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58092"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25073944"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2018.04.009"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111286"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2019.07.026"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0879-y"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70491","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.70491","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70491","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T13:51:03Z","timestamp":1780062663000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70491"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/jmv.70491"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70491","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2024-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70491"}}