{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:36:37Z","timestamp":1789421797849,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dengue pathogenesis involves immune\u2010driven inflammation that contributes to severe disease progression. This study assessed a machine learning model to identify a minimal, yet highly predictive biomarker set, aiming to support clinical decision\u2010making and patient triage. A total of 48 inflammatory mediators were quantified from plasma samples collected at admission from confirmed dengue patients, classified as either dengue without warning signs (DF) or dengue with warning signs\/severe dengue (DWS\/SD). A random forest approach was applied to identify the most predictive biomarkers associated with disease severity requiring hospitalization, based on admission\u2010time variables. Among the 48 immune mediators, 43 were differentially expressed in dengue patients versus healthy controls, and 26 showed significant differences between DF and DWS\/SD cases. Lymphocyte counts negatively correlated with IL\u20101RA, while liver enzymes showed positive correlations with HGF and SCGF\u2010beta; platelet counts also negatively correlated with these markers. Key severity\u2010associated markers included HGF, TNF\u2010beta, MIP\u20101\u2010beta, and SCGF\u2010beta. A model incorporating these markers and fever duration achieved nearly 80% accuracy in distinguishing DWS\/SD from DF cases, independent of clinical examination. The findings suggest that targeted cytokine profiling may guide early hospitalization decisions and ease healthcare burdens in dengue\u2010endemic regions.<\/jats:p>","DOI":"10.1002\/jmv.70511","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T10:39:17Z","timestamp":1753353557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Risk Stratification of Dengue Cases Requiring Hospitalization"],"prefix":"10.1002","volume":"97","author":[{"given":"Do Duc","family":"Anh","sequence":"first","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"Vietnamese German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Recker","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"Centre for Ecology and Conservation University of Exeter Penryn UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Trong","family":"The","sequence":"additional","affiliation":[{"name":"Vietnamese German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"},{"name":"108 Military Central Hospital Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Krishna","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"School of Health and Medical Sciences, Institute of Infection and Immunity City St George's University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter G.","family":"Kremsner","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 (CERMEL) Lambarene Gabon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Huu","family":"Song","sequence":"additional","affiliation":[{"name":"Vietnamese German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"},{"name":"108 Military Central Hospital Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9809-9883","authenticated-orcid":false,"given":"Thirumalaisamy P.","family":"Velavan","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"Vietnamese German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"},{"name":"Faculty of Medicine Duy Tan University Da Nang Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2830-8"},{"issue":"1","key":"e_1_2_11_3_1","first-page":"102","article-title":"Mortality Among Hospitalized Dengue Patients With Comorbidities in Mexico, Brazil, and Colombia","volume":"105","author":"Macias A. 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