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In the present study, we demonstrate that dengue infection modulates the expression of RBMX (an RNA\u2010binding protein) and miR\u2010133a. Viral infection elevates the expression of the RBMX gene while downregulates the level of miR\u2010133a. Additionally, Targetscan tool analysis shows that miR\u2010133a possesses a potential binding site in the 3\u2032UTR region of the RBMX gene, and our luciferase data indicate the miR\u2010133a\u2010mediated regulation of RBMX expression. Intriguingly, our time point study in Huh7 cells overexpressing the synthetic form of miR\u2010133a mimic and inhibitor indicates the convoluted interaction between miR\u2010133a and RBMX regulation during DENV infection. After 24\u2009h postinfection (hpi), miR\u2010133a significantly suppresses both the RBMX expression and viral RNA levels, acting as an antiviral agent by targeting the expression of the RBMX gene. Additionally, our immunoprecipitation result suggested the central role of DENV 3\u2032UTR in regulating the expression of both RBMX and miR\u2010133a. Furthermore, our study on RBMX Overexpression illuminates the vital function of RBMX protein in the DENV life cycle. Overexpression of the RBMX gene in ivermectin\u2010pretreated cells partially rescues viral replication. This comprehensive study explicates the dynamic miRNA\/RBPs regulatory axis during DENV pathogenesis.<\/jats:p>","DOI":"10.1002\/jmv.70543","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T11:40:27Z","timestamp":1754566827000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dynamic Interplay Between miR\u2010133a and RBMX During Dengue Virus Infection"],"prefix":"10.1002","volume":"97","author":[{"given":"Anjali","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Biotechnology All India Institute of Medical Sciences Ansari Nagar New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saumya","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Biotechnology All India Institute of Medical Sciences Ansari Nagar New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1731-5335","authenticated-orcid":false,"given":"Bhupendra","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Biotechnology All India Institute of Medical Sciences Ansari Nagar New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,7]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"217","article-title":"Genomics, Proteomics and Evolution of Dengue Virus","volume":"16","author":"Dwivedi V. 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