{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:44:40Z","timestamp":1790689480624,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\u03b2\u2010Coronaviruses hijack host factors to remodel host endo\u2010membranes to form double membrane vesicles (DMVs), which act as central hubs for the replication of viral genomes. Understanding the molecular mechanism underlying DMV formation is critical for developing effective antiviral strategies and has garnered significant attention. However, the host factors involved in DMV formation remain scanty. Here, we employed a bimolecular fluorescence complementation\u2010based proximity labeling (BiFCPL) strategy to investigate the proteome of DMVs generated by co\u2010expression of SARS\u2010CoV\u20102 NSP3 and NSP4. Our analysis identified 62 proteins with high confidence, among which five proteins were localized in the endoplasmic reticulum (ER), and were further confirmed to be recruited to DMVs through interactions with NSP3\/NSP4. Moreover, we demonstrated that the absence of GRAMD1B or TEX2 resulted in the formation of enlarged DMVs induced by either NSP3\/NSP4 or coronaviruses infection, and impaired coronaviruses replication as well. Collectively, our study concerning host\u2010virus interactions sheds light on novel host factors involved in DMV formation.<\/jats:p>","DOI":"10.1002\/jmv.70547","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T14:08:56Z","timestamp":1755785336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Spatial Proteomics Using BiFCPL Identifies Regulators of DMV Formation Involved in Coronavirus Replication"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8651-8545","authenticated-orcid":false,"given":"Maoge","family":"Zhou","sequence":"first","affiliation":[{"name":"Guangzhou National Laboratory Guangzhou Guangdong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinping","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangzhou National Laboratory Guangzhou Guangdong Province China"},{"name":"Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology Wuhan Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buyun","family":"Tian","sequence":"additional","affiliation":[{"name":"Guangzhou National Laboratory Guangzhou Guangdong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyue","family":"Wang","sequence":"additional","affiliation":[{"name":"National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing University of Posts and Telecommunications Beijing 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