{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:06:10Z","timestamp":1791986770891,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Flaviviruses such as dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) pose a major health burden in Vietnam, where overlapping clinical features and serological cross\u2010reactivity complicate accurate diagnosis and outbreak control. This study aimed to investigate circulating DENV serotypes and assess serological cross\u2010reactivity with other flaviviruses during the 2016 dengue outbreak in central Vietnam. Aretrospective study was conducted on 146 hospitalized dengue patients during the 2016 outbreak in Binh Dinh province. Laboratory diagnosis included NS1antigen testing, ELISA (IgM\/IgG), and real\u2010time RT\u2010PCR for DENV serotyping. IgM and IgG cross\u2010reactivity with five flaviviruses, including DENV, ZIKV, JEV, West Nile virus (WNV), and tick\u2010borne encephalitis virus (TBEV), and onealphavirus, chikungunya virus (CHIKV), was evaluated using ELISA. DENV\u20101 positive samples were further analysed by sequencing the capsid\u2010premembrane (CprM) gene. DENV\u20101 was the predominant serotype (86%), with all sequenced strains clustering within genotype I. Secondary infections were more frequent (64%) than primary infections (36%) and were associated with a significantly higher median age (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.003) and elevated hs\u2010CRP levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.029). Strong IgG cross\u2010reactivity was observed among flaviviruses, particularly DENV, JEV, WNV, and TBEV (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009&gt;\u20090.85), while ZIKV and CHIKV showed low seropositivity. Incontrast, IgM responses demonstrated greater virus specificity. Ten PCR\u2010negative cases showed broad serological reactivity, suggesting possible misdiagnosis or late\u2010stage infection. Our findings reveal that DENV\u20101 genotype I was the predominant serotype during the 2016 outbreak in central Vietnam. Extensive IgG cross\u2010reactivity among flaviviruses hinders serological diagnosis, highlighting the need for integrated molecular surveillance to ensure accurate outbreak response.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70569","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T14:27:10Z","timestamp":1755786430000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dominance of DENV\u20101 and Flavivirus Serological Cross\u2010Reactivity During the 2016 Dengue Outbreak in Vietnam"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7493-4717","authenticated-orcid":false,"given":"Do Duc","family":"Anh","sequence":"first","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen and German Center for Infection Research (DZIF) T\u00fcbingen Germany"},{"name":"Vietnamese \u2010 German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Huy","family":"Loc","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen and German Center for Infection Research (DZIF) T\u00fcbingen Germany"},{"name":"Vietnamese \u2010 German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang Van","family":"Tong","sequence":"additional","affiliation":[{"name":"Vietnamese \u2010 German Center for Medical Research (VG\u2010CARE) Hanoi Vietnam"},{"name":"Department Pathophysiology Vietnam Military Medical University Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Thi Huyen","family":"Dieu","sequence":"additional","affiliation":[{"name":"Binh Dinh Medical College Qui Nhon Gia Lai Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngo Thu","family":"Hang","sequence":"additional","affiliation":[{"name":"Department Pathophysiology Vietnam Military Medical University Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Huu","family":"Lanh","sequence":"additional","affiliation":[{"name":"Binh Dinh Hospital Qui Nhon Gia Lai Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi Hong","family":"Nhung","sequence":"additional","affiliation":[{"name":"Binh Dinh Medical College Qui Nhon Gia Lai Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter G.","family":"Kremsner","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of T\u00fcbingen and German Center for Infection Research (DZIF) T\u00fcbingen Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 (CERMEL) Lambarene Gabon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le 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