{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T03:26:59Z","timestamp":1781753219263,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Persistent high\u2010risk human papillomavirus (hHPV) infection, especially HPV\u201016, plays a central role in the development of high\u2010grade squamous intraepithelial lesions (HSIL). This study aimed to evaluate the performance of co\u2010testing (cytology and hHPV detection) in a real\u2010world cohort of men who have sex with men (MSM) and transgender women (TW) living with HIV. We conducted a prospective study (2017\u20132023) at a tertiary care center in Spain. MSM and TW living with HIV underwent screening with anal cytology and PCR\u2010based hHPV testing. High\u2010resolution anoscopy (HRA) with biopsy was performed in cases with abnormal cytology and\/or hHPV positivity. Clinical, epidemiological, and HIV\u2010related data were collected to identify risk factors for abnormal cytology and biopsy. 734 cytologies were performed in 380 participants. Abnormal cytology was found in 34%, most commonly ASCUS. hHPV was detected in 63.1% of samples; HPV\u201016 was the most prevalent genotype (19.4%), present in 60% of HSIL cytologies and 65% of AIN2\u20133 biopsies. Risk factors for abnormal cytology included nadir CD4\u2009&lt;\u2009200 cells\/\u03bcL (aOR 2.61), prior condylomas (aOR 2.66), and infection with any oncogenic HPV genotype (aOR 4.12). Among 91 HRAs, 29.6% showed AIN2\u20133% and 2.1% in situ carcinoma. HPV\u201016, HPV\u201052, and HPV\u201066 were most frequently associated with abnormal findings in the cytology. In conclusion, anal HPV infection was prevalent in MSM and TW living with HIV. The genotypes most frequently associated with abnormal cytology and histopathological findings were HPV\u201016, HPV\u201052, and HPV\u201066. These findings highlight the potential value of implementing co\u2010testing strategies in anal dysplasia screening for this high\u2010risk population.<\/jats:p>","DOI":"10.1002\/jmv.70596","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T13:37:11Z","timestamp":1757425031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Role of Human Papillomavirus Genotypes on Anal Squamous Intraepithelial Lesions Among Gay, Bisexual and Other Men Who Have Sex With Men Living With HIV: Beyond HPV\u201016"],"prefix":"10.1002","volume":"97","author":[{"given":"Aroa","family":"Villoslada","sequence":"first","affiliation":[{"name":"Hospital Universitari Son Ll\u00e0tzer. 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Internal Medicine (Infectious diseases) Palma Spain"},{"name":"Health Research, Institute of the Balearic Islands (IdISBa) Palma Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araceli","family":"Serrano","sequence":"additional","affiliation":[{"name":"Hospital Universitari Son Ll\u00e0tzer. Internal Medicine (Infectious diseases) Palma Spain"},{"name":"Health Research, Institute of the Balearic Islands (IdISBa) Palma Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Salom","sequence":"additional","affiliation":[{"name":"Hospital Universitari Son Ll\u00e0tzer. Internal Medicine (Infectious diseases) Palma Spain"},{"name":"Health Research, Institute of the Balearic Islands (IdISBa) Palma Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Collado","sequence":"additional","affiliation":[{"name":"Hospital Universitari Son Ll\u00e0tzer. 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Internal Medicine (Infectious diseases) Palma Spain"},{"name":"Health Research, Institute of the Balearic Islands (IdISBa) Palma Spain"},{"name":"Department of Medicine University of the Balearic Islands (UIB) Palma Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23744"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e3283023f78"},{"key":"e_1_2_10_4_1","first-page":"401","article-title":"The Increasing Incidence of Anal Cancer: Can it be Explained by Trends in Risk Groups?","volume":"71","author":"Van der Zee R. 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