{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:04:40Z","timestamp":1791227080304,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002462","name":"Chungnam National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002462","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The recent emergence of Japanese encephalitis virus genotype 5 (JEV5) has raised concerns regarding limited vaccine efficacy. Here, we report the construction of a full\u2010length infectious cDNA clone of JEV5 using a bacterial artificial chromosome (BAC) system, based on a South Korea isolate, K15P38 strain. The resulting clone enabled recovery of replication\u2010competent virus and was genetically distinguishable from the parental strain via engineered silent mutations. To extend its application, we introduced nanoluciferase and enhanced green fluorescent protein into the viral genome, generating reporter viruses suitable for replication tracking. Both reporter viruses supported real\u2010time quantification of viral replication and demonstrated utility in antiviral compound screening. Antiviral evaluation using the nucleoside analog NITD008 yielded EC\u2085\u2080 values consistent between reporter and classical plaque assays. These findings provide a reverse genetics platform for studying JEV5 and suggest applicability for genotype\u2010specific vaccine and antiviral research.<\/jats:p>","DOI":"10.1002\/jmv.70608","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T13:54:56Z","timestamp":1758030896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Japanese Encephalitis Virus Genotype 5 Infectious Clone and Reporter System for Antiviral Evaluation"],"prefix":"10.1002","volume":"97","author":[{"given":"Jae\u2010Yeon","family":"Park","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Chungnam National University Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Kamitani","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Host Defense Gunma University Graduate School of Medicine Gunma 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