{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:14:00Z","timestamp":1790356440475,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T00:00:00Z","timestamp":1759536000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Chandipura virus (CHPV), a neurotropic member of the Rhabdoviridae family, causes severe paediatric encephalitis outbreaks in India with high fatality rates and no approved antiviral therapies. In this study, lycorine, a plant\u2010derived alkaloid with established broad\u2010spectrum antiviral activity, was evaluated for its efficacy against CHPV. In vitro treatment with lycorine resulted in a\u2009&gt;\u20092.5\u2009log\u2081\u2080 reduction in viral, with minimal cytotoxicity and favourable selectivity indices across multiple cell lines. Time\u2010of\u2010addition assays demonstrated that lycorine exerts its antiviral effect during early stages of infection, without affecting the viral entry or egress. Quantitative RT\u2010PCR revealed a significant inhibition of CHPV positive\u2010strand RNA synthesis, indicating disruption of early replication steps. To elucidate the mechanism of action, molecular docking studies were performed using a structural model of CHPV L protein, based on high\u2010homology alignment with Vesicular Stomatitis Virus (VSV) polymerase. Docking and free energy calculations identified two high\u2010affinity lycorine\u2010binding sites within the RdRp catalytic domain, stabilized by hydrogen bonding and aromatic interactions. These findings suggest that lycorine may inhibit CHPV replication by targeting the viral RNA\u2010dependent RNA polymerase. Overall, this study highlights lycorine as a promising antiviral candidate against CHPV and potentially other neurotropic RNA viruses.<\/jats:p>","DOI":"10.1002\/jmv.70636","type":"journal-article","created":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T09:37:06Z","timestamp":1759570626000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lycorine Inhibits Chandipura Virus Replication In Vitro"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4657-8978","authenticated-orcid":false,"given":"Tanvi","family":"Agrawal","sequence":"first","affiliation":[{"name":"Centre for Immunobiology and Immunotherapy, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"},{"name":"Centre for Virus Research, Therapeutics and Vaccines, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Singh","sequence":"additional","affiliation":[{"name":"Centre for Tuberculosis Research, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gurleen","family":"Kaur","sequence":"additional","affiliation":[{"name":"Centre for Virus Research, Therapeutics and Vaccines, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiva","family":"Rathore","sequence":"additional","affiliation":[{"name":"Centre for Immunobiology and Immunotherapy, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harshita","family":"Sharma","sequence":"additional","affiliation":[{"name":"Centre for Immunobiology and Immunotherapy, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunjan","family":"Rajput","sequence":"additional","affiliation":[{"name":"Centre for Immunobiology and Immunotherapy, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sweety","family":"Samal","sequence":"additional","affiliation":[{"name":"Centre for Virus Research, Therapeutics and Vaccines, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2563-1971","authenticated-orcid":false,"given":"Amit","family":"Awasthi","sequence":"additional","affiliation":[{"name":"Centre for Immunobiology and Immunotherapy, Translational Health Science and Technology Institute NCR\u2010Biotech Science Cluster Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.70032"},{"key":"e_1_2_10_3_1","first-page":"395","article-title":"Chandipura Virus Encephalitis Outbreak Among Children in Nagpur Division, Maharashtra, 2007","volume":"132","author":"Gurav Y. 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