{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:45:48Z","timestamp":1791945948385,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Mpox, caused by the orthopoxvirus monkeypox virus (MPXV), has garnered worldwide attention during a global outbreak in 2022 and in an ongoing outbreak in Central Africa. Because of drug resistance and toxicity risks with current antiviral treatments, there is still a need for additional antivirals to treat mpox and other orthopoxvirus diseases. We developed in vitro screening assays using recombinant strains of vaccinia virus and MPXV to measure the antiviral potency of compounds, finding that adefovir dipivoxil and tenofovir alafenamide had potent anti\u2010orthopoxvirus activity and low toxicity. These data reinforce interest in repurposing nucleoside analogues as antivirals to treat poxvirus infections and provide a basis for high throughput screening and mechanistic and antiviral resistance studies.<\/jats:p>","DOI":"10.1002\/jmv.70669","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:46:28Z","timestamp":1761396388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Monkeypox Virus Is Inhibited by Nucleoside Analogues Including the Acyclic Phosphonates Adefovir and Tenofovir In Vitro"],"prefix":"10.1002","volume":"97","author":[{"given":"Jasper","family":"Lee","sequence":"first","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emerson Ailidh","family":"Boggs","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse","family":"Kilcullen","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Moriarty","sequence":"additional","affiliation":[{"name":"Poxvirus and Rabies Branch, National Center for Emerging Zoonotic Infectious Diseases, Centers for Disease Control and Prevention Division of High\u2010Consequence Pathogens and Pathology Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology Emory University School of Medicine Atlanta Georgia USA"},{"name":"Emory University School of Medicine, Emory Chemical Biology Discovery Center Atlanta Georgia USA"},{"name":"Winship Cancer Institute of Emory University Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haian","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology Emory University School of Medicine Atlanta Georgia USA"},{"name":"Emory University School of Medicine, Emory Chemical Biology Discovery Center Atlanta Georgia USA"},{"name":"Winship Cancer Institute of Emory University Atlanta Georgia USA"},{"name":"Department of Hematology and Medical Oncology Emory University Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuiyun","family":"Lan","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina L.","family":"Hutson","sequence":"additional","affiliation":[{"name":"Poxvirus and Rabies Branch, National Center for Emerging Zoonotic Infectious Diseases, Centers for Disease Control and Prevention Division of High\u2010Consequence Pathogens and Pathology Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panayampalli S.","family":"Satheshkumar","sequence":"additional","affiliation":[{"name":"Poxvirus and Rabies Branch, National Center for Emerging Zoonotic Infectious Diseases, Centers for Disease Control and Prevention Division of High\u2010Consequence Pathogens and Pathology Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip R.","family":"Tedbury","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5840-154X","authenticated-orcid":false,"given":"Stefan G.","family":"Sarafianos","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, Center for ViroScience and Cure Atlanta Georgia USA"},{"name":"Children's Healthcare of Atlanta Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,25]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"WHO Recommends New Name for Monkeypox Disease","author":"World Health Organization","year":"2023"},{"key":"e_1_2_9_3_1","unstructured":"Centers for Disease Control and Prevention (CDC). \u201cMonkeypox Outbreak Global Map \u201d (2022) https:\/\/www.cdc.gov\/poxvirus\/mpox\/response\/2022\/world-map.html."},{"key":"e_1_2_9_4_1","unstructured":"\u201cCenters for Disease Control and Prevention (CDC) \u201d U.S. Mpox Case Trends Reported to CDC accessed April 7 2023 https:\/\/www.cdc.gov\/poxvirus\/mpox\/response\/2022\/mpx-trends.html."},{"key":"e_1_2_9_5_1","unstructured":"\u201cMpox\u2014Democratic Republic of the Congo \u201d World Health Organization accessed August 31 2024 https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2024-DON522."},{"key":"e_1_2_9_6_1","unstructured":"\u201cFDA Approves Drug to Treat Smallpox \u201d US Food and Drug Administration accessed January 17 2023 https:\/\/www.fda.gov\/drugs\/news-events-human-drugs\/fda-approves-drug-treat-smallpox."},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00021-09"},{"key":"e_1_2_9_8_1","unstructured":"\u201cAntiviral Tecovirimat Safe but Did Not Improve Clade I Mpox Resolution in the Democratic Republic of the Congo \u201d National Institutes of Health accessed July 26 2025 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