{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:45:50Z","timestamp":1791945950103,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The profiles of spliced pgRNA variants in NAs\u2010treated HBV\u2010infected patients were still unclear, and the sensitivity and accuracy of quantification of HBV RNA or spliced pgRNA variants 1 (SP1) needs improvement. In this study, we identified the pattern of spliced pgRNA variants in HBV\u2010infected patients including treatment\u2010na\u00efve and NAs\u2010treatment with our established long\u2010read sequencing strategy. 16 reported spliced pgRNA and 41 novel spliced variants were identified by long\u2010read sequencing, and SP1 variant was the most abundant among all HBV spliced RNA, ranging from 35% to 62%. The proportion of spliced pgRNA\/total HBV RNA and SP1\/HBV RNA decreased after short\u2010term NAs treatment. Furthermore, we established an accurate assay for detection of serum HBV RNA and SP1 based on long\u2010read sequencing results, and evaluated the detection capability in HBV\u2010infected patients. A good consistency was observed in quantification of serum HBV RNA levels both in treatment\u2010na\u00efve and NAs treatment patients measured by primers sets 2312\/2413 and 1528\/1600 located outside the SP1 region. Finally, the proportion of SP1\/HBV RNA was associated with the degree of liver inflammation. Therefore, we determined the characteristics of spliced pgRNA and dynamic change in NAs\u2010treated patients by long\u2010read sequencing, and provide more information to support developing more accurate HBV RNA or SP1 variant detection assay.<\/jats:p>","DOI":"10.1002\/jmv.70676","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T14:20:27Z","timestamp":1761834027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Developing an Accurate Assay for Detection of Serum HBV RNA and SP1 Variant in CHB Based on Long\u2010Read Sequencing"],"prefix":"10.1002","volume":"97","author":[{"given":"Yueyue","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, 109#, Chongqing Medical University Chongqing Yuzhong District 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