{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:45:46Z","timestamp":1791945946280,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Esophageal varices (EV) are observed in 60%\u201385% of patients with decompensated cirrhosis at the time of diagnosis and in 30%\u201340% of patients with compensated cirrhosis. Hemorrhaging from these varices is a critical medical event and is associated with a mortality rate of approximately 40%. Therefore, it is essential to assess varices that show a risk for bleeding and then implement preventive measures. This urgency has sparked interest in several factors, including genetic polymorphisms, that influence the development of EV. To assess the prognostic value of specific single\u2010nucleotide polymorphisms (SNPs) in\n                    <jats:italic>ANGPT2 (rs2442598) and NOS3 (rs2070744)<\/jats:italic>\n                    genes involved in EVdevelopment. Four\u2010hundred fifty patients were enrolled: (1) 150 patients with hepatitis C (HCV)\u2010related cirrhosis and endocscopically detected varices (Group I), (2) 150 patients with HCV\u2010related cirrhosis and no varices (Group II), and 150 apparently healthy people as controls (Group III). Liver function tests, hepatitis viral markers, complete blood count {CBC}, abdominal ultrasonography, and detection of\n                    <jats:italic>ANGPT2<\/jats:italic>\n                    (\n                    <jats:italic>rs2442598<\/jats:italic>\n                    )\n                    <jats:italic>and NOS3 (rs2070744)<\/jats:italic>\n                    based on real\u2010time polymerase chain reaction were obtained for all participants. No significant differences in the genotype distribution or allele frequencies of\n                    <jats:italic>ANGPT2 (rs2442598) and NOS3 (rs2070744)<\/jats:italic>\n                    between patients with or without EV (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05) were found. However, significant differences were noted for\n                    <jats:italic>ANGPT2 rs2442598<\/jats:italic>\n                    in relation to the presence of varices, ascites, Child\u2013Pugh scores, hypertension (HTN), and levels of alanine and aspartate aminotransferase (ALT and AST, respectively;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), while other parameters did not show significant differences (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Similarly, for\n                    <jats:italic>NOS3 rs2070744<\/jats:italic>\n                    , significant differences were found in Child\u2013Pugh scores, HTN, and levels of ALT and AST (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The\n                    <jats:italic>ANGPT2 (rs2442598)<\/jats:italic>\n                    variant is a promising noninvasive indicator to confirm the absence of varices with portal hypertension to predict the absence of EV as a complication of cirrhosis.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70686","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:37:39Z","timestamp":1762274259000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association of\n                    <i>ANGPT2 and NOS3<\/i>\n                    Gene Variants With Esophageal Variceal Progression in HCV\u2010Induced Cirrhosis"],"prefix":"10.1002","volume":"97","author":[{"given":"Omnia S.","family":"Nabih","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology National liver institute, Menoufia university Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8970-0286","authenticated-orcid":false,"given":"Mohamed","family":"Abdel\u2010Samiee","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology National Liver Institute Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa A.","family":"Tahoon","sequence":"additional","affiliation":[{"name":"Departmentof Epidemiology and preventive medicine department National liver institute, Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaf","family":"Abozeid","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology National liver institute, Menoufia university Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7698-8326","authenticated-orcid":false,"given":"Heba","family":"Demerdash","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology National Liver Institute Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma Omar","family":"Khalil","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Microbiology and Immunology, National Liver Institute Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randa M.","family":"Seddik","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Faculty of Medicine Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa L.","family":"Helal","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Molecular Diagnostics National Liver Institute, Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gamalat A.","family":"El Gedawy","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and Molecular Diagnostics National Liver Institute, Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HalaA","family":"E. Khalil","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Immunology National Liver Institute, Menoufia University Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badawy W AboBakr.","family":"Yossef","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Faculty of Medicine Al\u2010Azhar University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8268-4538","authenticated-orcid":false,"given":"Ahmed B.","family":"Zaid","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology National liver institute hospital Menoufia university Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai I.","family":"Elashmawy","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology National liver institute, Menoufia university Shebin El\u2010kom Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.13426"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43066-022-00181-4"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.4132\/jptm.2023.05.24"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-017-9826-x"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43066-020-00025-z"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.giec.2006.01.019"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-24432-3_55"},{"key":"e_1_2_13_9_1","first-page":"15","article-title":"Portal Hypertension, an Overview","volume":"4","author":"Elmoghazy M.","year":"2019","journal-title":"Med J Viral Hepatitis"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43066-021-00159-8"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43066-022-00188-x"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25287"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43066-020-00038-8"},{"key":"e_1_2_13_14_1","first-page":"4725","article-title":"The Role of the eNOS G894T and T\u2010786C Gene Polymorphism in the Development of Ascites in Cirrhosis","volume":"20","author":"Yildirim O.","year":"2016","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.2217\/pgs-2018-0098"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.5604\/16652681.1198799"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.1003-9406.2012.01.018"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livres.2022.01.003"},{"key":"e_1_2_13_19_1","unstructured":"C. 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