{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:46:16Z","timestamp":1791945976649,"version":"4.3.4"},"reference-count":91,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:00:00Z","timestamp":1762560000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dengue virus (DENV) represents an escalating global public health threat, with rising incidence and expanding geographic distribution. Early and accurate diagnosis remains the cornerstone of effective outbreak management in the absence of universally accessible vaccines or antiviral therapies. Conventional diagnostic methods including viral nucleic acid detection, antigen\/antibody testing have achieved substantial progress but still face limitations in sensitivity, speed, and applicability within resource\u2010limited settings. Recent technological innovations are reshaping the diagnostic landscape. Advances in isothermal amplification, CRISPR\u2010based detection, and NGS have markedly enhanced the sensitivity, specificity, and rapidity of DENV detection. In addition, the incorporation of noninvasive\u00a0samples such as saliva and urine, together with home\u2010based and POCT systems, is expanding diagnostic accessibility. The WHO's REASSURED framework further provides essential guidance for developing affordable, rapid, and field\u2010deployable diagnostic tools. This review summarizes current DENV diagnostic strategies, spanning nucleic acid and serological detection, and highlights recent innovations that integrate these approaches into tiered diagnostic systems. By combining technological advancement with clinical and public health applicability, these strategies hold great promise for strengthening early detection, improving outbreak surveillance, and supporting the global effort toward dengue elimination.<\/jats:p>","DOI":"10.1002\/jmv.70688","type":"journal-article","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:15:42Z","timestamp":1762604142000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrative Advances in Dengue Virus Diagnostics: From Technological Innovation to Tiered Diagnostic Systems"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6076-7711","authenticated-orcid":false,"given":"Yaoqiang","family":"Shi","sequence":"first","affiliation":[{"name":"Institute of Basic and Clinical Medicine, The First People's Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Basic and Clinical Medicine, The First People's Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Basic and Clinical Medicine, The First People's Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5382-5725","authenticated-orcid":false,"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Basic and Clinical Medicine, The First People's Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province Faculty of Life Science and Technology, Kunming University of Science and Technology Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhu","family":"Song","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province Faculty of Life Science and Technology, Kunming University of Science and Technology Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Basic and Clinical Medicine, The First People's Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-021-00824-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-022-01013-8"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)02576-X"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14040768"},{"key":"e_1_2_10_6_1","unstructured":"Organization WH \u201cGlobal Dengue Surveillance \u201d2024."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2542-5196(25)00059-2"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2149\/tmh.2011-S05"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fepid.2024.1342723"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43247-024-01639-6"},{"key":"e_1_2_10_11_1","unstructured":"Organization WH \u201cDengue and Severe Dengue \u201d2024."},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14061228"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03682-5"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23084197"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.707287"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.34133\/jbioxresearch.0034"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvacx.2024.100604"},{"key":"e_1_2_10_18_1","volume-title":"In: StatPearls. 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