{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:46:18Z","timestamp":1791945978500,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Cytomegalovirus (CMV) poses significant risks to infants and immunocompromised individuals. This study investigated the prevalence, associated risk factors, and phylogenetic profile of CMV DNA in the breast milk of lactating mothers in Khyber Pakhtunkhwa (KP), Pakistan. Breast milk samples (\n                      <jats:italic>n\u2009<\/jats:italic>\n                      =\u2009406) were collected from mothers across four districts. Demographic factors were recorded, and CMV DNA was detected by conventional and nested PCR targeting the\n                      <jats:italic>UL71<\/jats:italic>\n                      gene. Sanger sequencing was performed on the\n                      <jats:italic>UL71<\/jats:italic>\n                      amplicons, and phylogenetic analysis was carried out using MEGA 7. CMV DNA was detected in 77\/406 samples (18.97%), with higher prevalence among younger mothers, women from lower\u2010income households, and those living in remote areas. Education level correlated positively with CMV awareness.\n                      <jats:italic>UL71<\/jats:italic>\n                      sequences obtained from positive samples clustered closely with known Asian isolates; however, we failed to detect sequence variation within the\n                      <jats:italic>UL71<\/jats:italic>\n                      region using Sanger sequencing. These findings do not exclude genetic diversity elsewhere in the CMV genome or the presence of minor variants. CMV is prevalent among lactating mothers in KP, particularly in socioeconomically disadvantaged groups. Rather than claiming broad genetic conservation, we report that no variation was detected in the sequenced\n                      <jats:italic>UL71<\/jats:italic>\n                      region by Sanger sequencing; whole\u2010genome or deep sequencing would be required to assess genome\u2010wide diversity. These results underscore the need for continued surveillance and targeted public health interventions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.70698","type":"journal-article","created":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T14:14:26Z","timestamp":1763302466000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence, Risk Factors, and Phylogeny of Cytomegalovirus DNA in Breast Milk of Lactating Mothers"],"prefix":"10.1002","volume":"97","author":[{"given":"Faryal","family":"Saad","sequence":"first","affiliation":[{"name":"Department of Zoology Abdul Wali Khan University Mardan Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumaira","family":"Shams","sequence":"additional","affiliation":[{"name":"Department of Zoology Abdul Wali Khan University Mardan Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan","family":"Ayaz","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Life Sciences Abdul Wali Khan University Mardan Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noor Ul","family":"Akbar","sequence":"additional","affiliation":[{"name":"Department of Zoology Kohat University of Science and Technology Kohat Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asar","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Zoology Abdul Wali Khan University Mardan Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mourad","family":"Ben Said","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet University of Manouba Manouba Tunisia"},{"name":"Department of Basic Sciences, Higher Institute of Biotechnology of Sidi Thabet University of Manouba Manouba Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa A.","family":"Abdel\u2010Maksou","sequence":"additional","affiliation":[{"name":"Research Chair of Biomedical Applications of Nanomaterials, Department of Biochemistry, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Alamri","sequence":"additional","affiliation":[{"name":"Biochemistry Department, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salman","family":"Alrokayan","sequence":"additional","affiliation":[{"name":"Research Chair of Biomedical Applications of Nanomaterials, Department of Biochemistry, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"El\u2010Tayeb","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6971-0957","authenticated-orcid":false,"given":"Farhad","family":"Badshah","sequence":"additional","affiliation":[{"name":"Livestock and Poultry Multi\u2010Omics of MARA, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen China"},{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.33899\/rjs.2018.159336"},{"issue":"2","key":"e_1_2_12_3_1","first-page":"1","article-title":"Relationship Between Increases Anticardiolipin Titer With CMV Infection in Pregnant Women","volume":"12","author":"Abbas N. 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