{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:46:16Z","timestamp":1791945976582,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Human T\u2010lymphotropic virus 1 (HTLV\u20101)\u2010associated myelopathy\/tropical spastic paraparesis (HAM\/TSP) is a progressive neurodegenerative disease affecting motor and sensory functions. While alterations in cerebrospinal fluid (CSF) have been used to identify disease biomarkers, the effects of such modified CSF on CNS cells remain unexplored. This study compared the effects of pools of CSF from HTLV\u20101 asymptomatic carriers (HAC) and HAM\/TSP patients\u2014categorized by disease progression as: very slow (HAMvs), typical (HAMt), and rapid (HAMr)\u2014on the glioblastoma cell line U87\u2010MG, a cellular model often used to study neurodegenerative diseases. RNA sequencing of U87\u2010MG cells treated with a pool of CSFs from HAMr patients revealed a significant downregulation of transcription of mitochondrial genes after 24\u2009h of treatment. Confocal microscopy showed phenotypical changes in the mitochondrial network: glioblastoma cells exposed to pooled HAMr CSF exhibited a less complex network compared to other patient groups. Despite these changes, U87\u2010MG cells treated with CSF from HTLV\u20101\u2010infected donors with distinct neurological outcomes presented similar mitochondrial oxygen consumption. In conclusion, these findings show that pooled HAMr CSF induces mitochondrial stress in glioblastoma cells, suggesting that CSF alterations may participate in rapidly progressing HAM\/TSP pathogenesis.<\/jats:p>","DOI":"10.1002\/jmv.70711","type":"journal-article","created":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T14:21:13Z","timestamp":1763302873000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cerebrospinal Fluid From Patients With HTLV\u20101\u2010associated Myelopathy\/Tropical Spastic Paraparesis (HAM\/TSP) With Rapid Evolution Affects Mitochondrial DNA Transcription and Network Organization in Human Glioblastoma Cells"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7554-1093","authenticated-orcid":false,"given":"Yago C\u00f4rtes Pinheiro","family":"Gomes","sequence":"first","affiliation":[{"name":"Instituto Nacional de Infectologia Evandro Chagas (INI), 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