{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T08:04:06Z","timestamp":1771315446196,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000589","name":"Chief Scientist Office","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000589","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    High risk human papillomaviruses (hrHPVs) cause most cervical cancers. Cervical screening programmes aim to identify precancerous disease using detection of hrHPV nucleic acid using clinician\u2010taken liquid\u2010based cytology (LBC) samples, or increasingly, self\u2010taken samples (STSs). However, STSs are incompatible with traditional cytology triage. Therefore, development of novel triage tests is essential. Quantification of cellular and viral mRNA biomarkers is one option, but little is known about mRNA quality in STSs. We extracted RNA from two sets of STSs (reflecting separate sampling devices) from the Scottish HPV Archive (SHA) and the Swedish Cervical Cytology Biobank (SCCB). We investigated whether the\n                    <jats:italic>ACTB<\/jats:italic>\n                    ,\n                    <jats:italic>GAPDH<\/jats:italic>\n                    and\n                    <jats:italic>p16<\/jats:italic>\n                    RNAs could be amplified by reverse transcription quantitative PCR (RT\u2010qPCR). RNA was degraded in both sets of samples, but samples from the Scottish HPV Archive were generally suitable for RT\u2010qPCR analysis, while samples from the SCCB were mostly unsuitable. Genomic DNA contamination was detected in 11.6% of samples. The quantity and quality of RNA derived from STSs was unaffected by storage of the original sample at \u221270\u00b0C for a period of 1 year. These data suggest that feasibility of utilising STSs for mRNA expression work is device\u2010dependent and that optimisation of collection and storage systems is warranted.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70737","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T11:15:44Z","timestamp":1765192544000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Viability of Self\u2010Taken Vaginal Swab Samples for RNA\u2010Based Biomarker Analysis in Cervical Disease"],"prefix":"10.1002","volume":"97","author":[{"given":"Harry","family":"Scott","sequence":"first","affiliation":[{"name":"MRC\u2010University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow Garscube Estate Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Stevenson","sequence":"additional","affiliation":[{"name":"MRC\u2010University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow Garscube Estate Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7169-9080","authenticated-orcid":false,"given":"Daniel","family":"Mair","sequence":"additional","affiliation":[{"name":"MRC\u2010University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow Garscube Estate Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William M.","family":"Rooney","sequence":"additional","affiliation":[{"name":"MRC\u2010University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow Garscube Estate Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"McMahon","sequence":"additional","affiliation":[{"name":"Institute for Regeneration and Repair University of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2498-5206","authenticated-orcid":false,"given":"Laila Sara Arroyo","family":"M\u00fchr","sequence":"additional","affiliation":[{"name":"Center for Cervical Cancer Elimination, Department of Clinical Science, Intervention and Technology, Karolinska Institutet F56 Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"Cuschieri","sequence":"additional","affiliation":[{"name":"Scottish HPV Reference Laboratory, Royal Infirmary of Edinburgh 51 Little France Crescent NHS Lothian Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7140-8279","authenticated-orcid":false,"given":"Sheila V.","family":"Graham","sequence":"additional","affiliation":[{"name":"MRC\u2010University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow Garscube Estate Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0969141318767471"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2019.105953"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12939-021-01548-6"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/jfprhc-2014-101082"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33967"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2021.106900"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.16352"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-025-04219-3"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-03014-6"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-023-02277-z"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-3251"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822009000100001"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2018.08.006"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2012.0036"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00869.x"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.5.2989-2997.1995"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430422"},{"key":"e_1_2_12_19_1","doi-asserted-by":"crossref","unstructured":"M. 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