{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T18:46:23Z","timestamp":1769539583895,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Herpes simplex virus type 1 (HSV\u20101) is among the most common pathogens in the human population. Primary infection and reactivation of the virus can cause serious diseases and death. The drug resistance to all approved anti\u2010HSV agents poses a significant challenge, especially for immunocompromised patients. This study focuses on some details of the dimeric bisbenzimidazole\n                    <jats:bold>DB<\/jats:bold>\n                    <jats:sub>\n                      <jats:bold>2<\/jats:bold>\n                    <\/jats:sub>\n                    <jats:bold>A(7)<\/jats:bold>\n                    synthesis and its antiviral properties of against both HSV\u20101 sensitive and resistant acyclovir isolates. Optimization of the synthesis of\n                    <jats:bold>DB<\/jats:bold>\n                    <jats:sub>\n                      <jats:bold>2<\/jats:bold>\n                    <\/jats:sub>\n                    <jats:bold>A(7)<\/jats:bold>\n                    resulted in an increase in the total yield from 20% to 44%, reduction of labor intensity and removal of highly toxic and carcinogenic hydrazine. The\n                    <jats:bold>DB<\/jats:bold>\n                    <jats:sub>\n                      <jats:bold>2<\/jats:bold>\n                    <\/jats:sub>\n                    <jats:bold>A(7)<\/jats:bold>\n                    binding to thymus DNA was confirmed by a combination of physicochemical methods including calculation of the binding constant.\n                    <jats:bold>DB<\/jats:bold>\n                    <jats:sub>\n                      <jats:bold>2<\/jats:bold>\n                    <\/jats:sub>\n                    <jats:bold>A(7)<\/jats:bold>\n                    demonstrated the in vitro capacity to inhibit viral activity, both before and after cell infection, and effectively inactivated cell\u2010free viruses. Gene expression analysis by RT\u2010PCR revealed a significant reduction in the transcription of the gene encoding the immediate early ICP0 HSV\u20101 protein, suggesting a mechanism of action different from that of acyclovir. We first demonstrated the in vivo preventive effect of\n                    <jats:bold>DB<\/jats:bold>\n                    <jats:sub>\n                      <jats:bold>2<\/jats:bold>\n                    <\/jats:sub>\n                    <jats:bold>A(7)<\/jats:bold>\n                    towards lethal skin HSV\u20101 infection in mice as well as its capacity to protect animals from lethal genital herpes. The data obtained implied the potential of the synthesized\n                    <jats:bold>DB<\/jats:bold>\n                    <jats:sub>\n                      <jats:bold>2<\/jats:bold>\n                    <\/jats:sub>\n                    <jats:bold>A(7)<\/jats:bold>\n                    as a new antiviral agent against the lethal HSV\u20101 infection.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70758","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:27:48Z","timestamp":1767007668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dimeric Bisbenzimidazole DB\n                    <sub>2<\/sub>\n                    A(7) Protects Mice From Lethal Genital Herpes and Herpetic Skin Lesions"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1961-9789","authenticated-orcid":false,"given":"Natalia A.","family":"Demidova","sequence":"first","affiliation":[{"name":"Gamaleya National Research Centre for Epidemiology and Microbiology Ministry of Health of the Russian Federation Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4147-8444","authenticated-orcid":false,"given":"Regina R.","family":"Klimova","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Centre for Epidemiology and Microbiology Ministry of Health of the Russian Federation Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5254-9252","authenticated-orcid":false,"given":"Albert F.","family":"Arutyunyan","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasiliy S.","family":"Koval","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey A.","family":"Kostyukov","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir A.","family":"Kuzmin","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena A.","family":"Shubina","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Centre for Epidemiology and Microbiology Ministry of Health of the Russian Federation Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruslan 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