{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T19:06:20Z","timestamp":1769540780728,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This multicenter clinical study evaluated 17 common pathogens in 1922 pharyngeal swab samples, comparing the diagnostic performance of polymerase chain reaction coupled with quantum dot fluorescence analysis (PCR\u2010QDFA) with that of clinically routine quantitative real\u2010time reverse transcription polymerase chain reaction (qRT\u2010PCR). The results were validated using Sanger sequencing as the gold standard. Our results showed that among the samples with single\u2010pathogen infections (1037 cases, 53.95%), the three most frequently detected pathogen were influenza A virus (IAV) (296 cases, 15.40%), severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) (164 cases, 8.53%), and\n                    <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    (94 cases, 4.89%). Similarly, among the samples with co\u2010infections of two or more pathogens (382 cases, 19.88%), the three most frequently detected pathogens were IAV (148 cases, 7.70%), SARS\u2010CoV\u20102 (107 cases, 5.57%), and\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    (67 cases, 3.49%). In the comprehensive evaluation of 17 respiratory pathogens, PCR\u2010QDFA demonstrated comparable diagnostic performance to qRT\u2010PCR, with an overall sensitivity of 99.78% (99.44%\u201399.92%) (vs. qRT\u2010PCR: 99.82% [99.48%\u201399.94%]) and specificity of 99.94% (99.90%\u201399.96%) (vs. qRT\u2010PCR: 99.95% [99.91%\u201399.97%]). PCR\u2010QDFA offers significant operational advantages, including high\u2010throughput capacity (96 samples per run) and lower cost.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70759","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T06:43:13Z","timestamp":1766990593000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Multicenter Clinical Evaluation of Polymerase Chain Reaction Coupled With Quantum Dot Fluorescence Analysis and Quantitative Real\u2010Time Reverse Transcription Polymerase Chain Reaction in the Diagnosis of Pathogens in Patients With Suspected Respiratory Tract Infections"],"prefix":"10.1002","volume":"98","author":[{"given":"Xinghan","family":"Huang","sequence":"first","affiliation":[{"name":"The Fourth School of Clinical Medicine, Hangzhou First People's Hospital Zhejiang Chinese Medical University Hangzhou Zhejiang China"},{"name":"Affiliated 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