{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:56:46Z","timestamp":1784037406221,"version":"3.55.0"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome (ME\/CFS) is a debilitating disease with unknown etiology and heterogeneous symptomology for which there are no validated tests for definitive diagnosis. We examined 873 longitudinal serum samples from ME\/CFS patients (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200940) and 378 from healthy control individuals (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200916) for differences in human herpesvirus and endogenous retrovirus\u2010K (HERV\u2010K) dUTPase IgG antibodies by ELISA. The results of this study demonstrate a significant increase in dUTPase IgG antibodies to the herpesviruses Epstein\u2010Barr virus (EBV), human herpesvirus\u20106 (HHV\u20106) and varicella zoster virus (VZV) in ME\/CFS compared to healthy\u2010controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Notably, 72.5% (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200929) of ME\/CFS patients simultaneously co\u2010expressed antibodies to multiple herpesvirus and HERV\u2010K dUTPases compared to 31% (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) of the healthy controls. Chi\u2010square test analysis showed strong associations for EBV, HHV\u20106 and VZV dUTPase antibodies seropositivity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and Spearman correlation analysis revealed significant positive associations of EBV and HHV\u20106 dUTPase IgG antibodies with fatigue. Further examination of the distribution of dUTPase antibodies across fatigue severity groups show that heightened dUTPase IgG levels cluster with ME\/CFS patients exhibiting moderate and severe fatigue. These findings highlight the importance of examining herpesvirus dUTPase IgG across severity groups in aiding with current challenges for stratifying ME\/CFS patients due to the heterogeneity in symptomology.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70769","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T11:40:17Z","timestamp":1766749217000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chronic Reactivation of Persistent Human Herpesviruses EBV, HHV\u20106 and VZV and Heightened Anti\u2010dUTPase IgG Antibodies Are a Recurrent Hallmark in Post\u2010Infectious ME\/CFS and is Associated With Fatigue"],"prefix":"10.1002","volume":"98","author":[{"given":"Irene Mena","family":"Palomo","sequence":"first","affiliation":[{"name":"Biomedical Sciences Graduate Program The Ohio State University College of Medicine Columbus Ohio USA"},{"name":"Institute for Behavioral Medicine Research (IBMR) The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Cox","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Graduate Program The Ohio State University College of Medicine Columbus Ohio USA"},{"name":"Institute for Behavioral Medicine Research (IBMR) The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marshall V.","family":"Williams","sequence":"additional","affiliation":[{"name":"Institute for Behavioral Medicine Research (IBMR) The Ohio State University Wexner Medical Center Columbus Ohio USA"},{"name":"Department of Cancer Biology and Genetics (CBG) The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2560-5367","authenticated-orcid":false,"given":"Maria Eugenia","family":"Ariza","sequence":"additional","affiliation":[{"name":"Institute for Behavioral Medicine Research (IBMR) The Ohio State University Wexner Medical Center Columbus Ohio USA"},{"name":"Department of Cancer Biology and Genetics (CBG) The Ohio State University Wexner Medical Center Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,26]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Encephalomyelitis\/Chronic Fatigue Syndrome: Redefining an illness","author":"Institute of Medicine of the National Academies","year":"2015"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-121-12-199412150-00009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1300\/J092v11n01_02"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2011.02428.x"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Myalgic Encephalomyelitis\/Chronic 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