{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:38Z","timestamp":1786977998628,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Epstein\u2010Barr virus (EBV) acquired in childhood is asymptomatic, with the tonsils as the entry site to infect B lymphocytes and establish latency. Little is known about whether the microbiota influences EBV persistent infection. We aimed to characterize the tonsil microbiome with different EBV latency types. A cohort of 60 children with tonsillar hyperplasia were included. EBV latency types (0\/I\/II\/III) and lytic phase were determined by immunohistochemistry. 16S NGS of lymphoid tonsillar tissue was carried out with the Illumina platform. Bioinformatic analysis was carried out with QIIME2 2023.2 software and the R studio packages Phyloseq and DESeq 2. Absolute quantification of\n                    <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    and its subspecies was conducted using qPCR. A minor \u03b1\u2010diversity was observed in the latency III group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0014). Therefore, the reference employed for differential abundance analysis was the latency III group. Latency III group was characterized by\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    when compared with non\u2010infected (LFC\u2009=\u200943.81,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20092.03E\u201010), latency 0 (LFC\u2009=\u200923.20,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20092.16E\u201005); latency I (LFC\u2009=\u200923.21,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20091.36E\u201007), and latency II (LFC\u2009=\u200938.03,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20097.36E\u201019). In contrast,\n                    <jats:italic>Prevotella histicola<\/jats:italic>\n                    was decreased (LFC\u2009=\u2009\u221226.06,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0004). Latency group III with lytic phase was characterized by\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    (LFC\u2009=\u200926.74,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20095.30E\u201005).\n                    <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    subspecies\n                    <jats:italic>vicentii<\/jats:italic>\n                    had a higher copy number within latency group III (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.020). We found that a microbiome signature in lymphoid tonsillar tissue is related to EBV infection, where\n                    <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    subspecies could play a crucial role in latency III and the lytic phase of EBV. This study provides the first evidence that the tonsil microbiome varies with EBV stages, a finding relevant to EBV\u2010associated diseases.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70808","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T08:55:24Z","timestamp":1768899324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tonsillar Microbial Signature of\n                    <i>Fusobacterium nucleatum<\/i>\n                    Associated With Epstein\u2010Barr Virus Latency"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7007-1291","authenticated-orcid":false,"given":"Norma Paola","family":"Aguirre Morgado","sequence":"first","affiliation":[{"name":"Doctorado en Microbiolog\u00eda M\u00e9dica, Departamento de Microbiolog\u00eda y Patolog\u00eda Centro Universitario de Ciencias de la Salud (CUCS) Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Laura","family":"Pereira Su\u00e1rez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en C\u00e1ncer e Infecciones (IICI) CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"},{"name":"Departamento de Microbiolog\u00eda y Patolog\u00eda CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Chabay","sequence":"additional","affiliation":[{"name":"Instituto Multidisciplinario de Investigaciones en Patolog\u00edas Pedi\u00e1tricas (IMIPP) CONICET\u2010GCBA Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3016-4295","authenticated-orcid":false,"given":"Marcela","family":"Pe\u00f1a Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Patolog\u00eda CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"},{"name":"Laboratorio de Diagn\u00f3stico de Enfermedades Emergentes y Reemergentes (LaDEER) CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Eugenia","family":"Amarillo","sequence":"additional","affiliation":[{"name":"Instituto Multidisciplinario de Investigaciones en Patolog\u00edas Pedi\u00e1tricas (IMIPP) CONICET\u2010GCBA Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Mangiaterra","sequence":"additional","affiliation":[{"name":"Instituto Multidisciplinario de Investigaciones en Patolog\u00edas Pedi\u00e1tricas (IMIPP) CONICET\u2010GCBA Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesly","family":"Bueno Urquiza","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Patolog\u00eda CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4601-4574","authenticated-orcid":false,"given":"Alina Xcaret","family":"Rubio S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Doctorado en Microbiolog\u00eda M\u00e9dica, Departamento de Microbiolog\u00eda y Patolog\u00eda Centro Universitario de Ciencias de la Salud (CUCS) Universidad de Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4749-2498","authenticated-orcid":false,"given":"Natali","family":"Vega Maga\u00f1a","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en C\u00e1ncer e Infecciones (IICI) CUCS, Universidad de Guadalajara Guadalajara M\u00e9xico"},{"name":"Departamento de Microbiolog\u00eda 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