{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T21:12:49Z","timestamp":1792530769651,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The global impact of pandemics has intensified the demand for rapid, cost\u2010effective, sensitive, and reliable POCT methods. Herein, we developed a multiplex pathogen detection system based on a dual\u2010drive microfluidic chip, enabling simultaneous detection of RSV, FluA, FluB, and SARS\u2010CoV\u20102 antigens from a single sample via parallel\u2010arranged detection units within microchannels. Monoclonal antibodies targeting conserved viral proteins were cross\u2010paired to identify optimal pairs. The chip's labeling and reference regions were designed, and key parameters were optimized to enhance performance. Cut\u2010off values were established using nasopharyngeal swab samples from healthy donors, and assay performance was rigorously validated. Finally, the concordance between the multiplex chip and GICA and RT\u2010PCR was evaluated. The optimized process ultimately yielded a chip design featuring a labeling region with four antiviral monoclonal antibody\u2010conjugated fluorescent microspheres, four test regions each coated with corresponding antiviral monoclonal antibodies, and a dual\u2010antibody reference region for signal normalization. The developed assay requires only 35\u2009\u03bcL of sample and completes detection within 5\u2009min. It demonstrated high repeatability, with coefficients of variation ranging from 9.48% to 13.37%. The detection limits were 0.625\u2009ng\/mL for RSV, 2.5\u2009ng\/mL for FluA, 5\u2009ng\/mL for both FluB and SARS\u2010CoV\u20102. Validation studies showed perfect agreement with GICA (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009132, Kappa\u2009=\u20091) and strong consistency with RT\u2010PCR (Kappa\u2009=\u20090.74\u20130.90,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200942). A novel POCT method has been successfully established for the simultaneous detection of four respiratory virus antigens. This system shows great potential as a reliable and efficient analytical tool for large\u2010scale epidemic screening.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70811","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T17:23:57Z","timestamp":1768843437000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of a Point\u2010of\u2010Care Testing Method for Rapid Detection of Multiple Respiratory Virus Antigens Based on a Dual\u2010Drive Microfluidic Chip"],"prefix":"10.1002","volume":"98","author":[{"given":"Xiaohui","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology, School of Medical Technology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Second Hospital of Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Medical Technology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josh Zixi","family":"Lee","sequence":"additional","affiliation":[{"name":"Beijing MicVic Biotech Co. Ltd. Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Tianjin Children's Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Tianjin Children's Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Second Hospital of Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Medical Technology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1632-4598","authenticated-orcid":false,"given":"Huiqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Medical Technology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, School of Medical Technology Tianjin Medical University Tianjin 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