{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:04:54Z","timestamp":1785524694042,"version":"3.56.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014738","name":"Vision Group on Science and Technology","doi-asserted-by":"publisher","award":["GRDno.1119"],"award-info":[{"award-number":["GRDno.1119"]}],"id":[{"id":"10.13039\/501100014738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Kyasanur Forest Disease (KFD), commonly known as \u2018monkey fever,\u2019 is a highly neglected tropical disease caused by the Kyasanur Forest Disease Virus (KFDV). KFD is endemic to the Western Ghats of Karnataka, India, with seasonal outbreaks during December to June every year. As there is no standard treatment regime, KFD can be fatal with a mortality rate of 2%\u201310%. Currently, KFD is detected through a non\u2010specific IgM\u2010ELISA followed by RT\u2010PCR, which often delays diagnosis, leading to increased disease severity and even death. To address this, we focused on developing a specific antigen\u2010based KFD detection. The KFDV Envelope Domain III (EDIII) and Non\u2010Structural 1 (NS1) proteins were chosen as detection markers, cloned, and expressed using pET28a(+) vector in BL\u201021 (Rosetta)\n                    <jats:italic>E. coli<\/jats:italic>\n                    and purified. These proteins were used to raise polyclonal antibodies in rabbits and the antibody titre was found to be 1:256\u2009000 and 1:512\u2009000 against rEDIII and rNS1 proteins, respectively. Importantly, these polyclonal antibodies showed no cross\u2010reactivity against corresponding dengue virus EDIII and NS1 proteins. Using polyclonal antibodies against rEDIII, we developed sandwich ELISA for the specific detection of KFD, which has demonstrated high specificity and sensitivity. Further, anti\u2010rEDIII polyclonal antibodies also detected full\u2010length KFDV\u2010E protein expressed in mammalian cells, confirming the antibody specificity for the native viral antigen.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70820","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T11:51:33Z","timestamp":1769255493000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of Recombinant Envelope Domain III as a Diagnostic Antigen for the Specific Detection of Kyasanur Forest Disease"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3701-1689","authenticated-orcid":false,"given":"Sayad","family":"Hafeez","sequence":"first","affiliation":[{"name":"Department of PG Studies and Research in Microbiology, Kuvempu University Jnana Sahyadri Shankaraghatta Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9867-8917","authenticated-orcid":false,"given":"Rajeshwara","family":"Achur","sequence":"additional","affiliation":[{"name":"Department of PG Studies and Research in Biochemistry, Kuvempu University Jnana Sahyadri Shankaraghatta Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7962-4979","authenticated-orcid":false,"given":"Vivek","family":"Vijay","sequence":"additional","affiliation":[{"name":"Molecular Bioassay Laboratory Institute of Advanced Virology (IAV) Thiruvananthapuram Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6345-3590","authenticated-orcid":false,"given":"Easwaran","family":"Sreekumar","sequence":"additional","affiliation":[{"name":"Molecular Bioassay Laboratory Institute of Advanced Virology (IAV) Thiruvananthapuram Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4680-0383","authenticated-orcid":false,"given":"Asha","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"School of Life Sciences JSS Academy of Higher Education and Research (AHER) Mysuru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4571-8716","authenticated-orcid":false,"given":"Suchetha","family":"Kumari","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Srinivas Institute of Medical Science and Research Centre, Srinivas University Mangaluru, D. K Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4736-3429","authenticated-orcid":false,"given":"N. B.","family":"Thippeswamy","sequence":"additional","affiliation":[{"name":"Department of PG Studies and Research in Microbiology, Kuvempu University Jnana Sahyadri Shankaraghatta Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.495"},{"key":"e_1_2_11_3_1","first-page":"341","article-title":"Summary of Preliminary Report of Investigations of the Virus Research Centre on an Epidemic Disease Affecting Forest Villagers and Wild Monkeys of Shimoga District, Mysore","volume":"11","author":"Work T. 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