{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:04:43Z","timestamp":1785524683136,"version":"3.56.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE\u2010HS) is an intractable form of epilepsy involving the hippocampus, and temporal lobectomy remains an effective treatment. Human herpesvirus 6B (HHV\u20106B) establishes latency in the hippocampus and may contribute to MTLE\u2010HS pathogenesis by altering host gene expression; however, transcriptomic data from healthy controls remain limited. This study investigated the role of HHV\u20106B to MTLE\u2010HS pathogenesis by analyzing gene expression in resected hippocampal tissues. Samples were collected from 12 to 43 HHV\u20106 DNA\u2010positive and \u2010negative patients, respectively, and three controls. RNA sequencing was performed on eight representative samples, followed by RT\u2010qPCR validation of nine selected genes in 58 samples. RNA sequencing identified 600 differentially expressed genes (210 upregulated, 390 downregulated) between HHV\u20106B\u2010positive MTLE\u2010HS and controls. Pathway enrichment analysis revealed involvement of synaptic signaling and inflammatory responses, with prostaglandin biosynthesis specifically upregulated in HHV\u20106B\u2010positive tissues. Two genes were significantly upregulated in HHV\u20106B\u2010positive compared with HHV\u20106B\u2010negative samples. RT\u2010qPCR confirmed elevated cholesterol 25\u2010hydroxylase and interleukin 1 beta expression in HHV\u20106 DNA\u2010positive samples (both\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.031). These findings suggest that HHV\u20106B may contribute to MTLE\u2010HS pathogenesis by modulating the expression of host inflammatory genes, supporting a role for neuroinflammation and the potential benefits of anti\u2010inflammatory therapies.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70845","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T12:29:39Z","timestamp":1771244979000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hippocampal Gene Expression in Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis: Assessment of Human Herpesvirus 6B DNA Status"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3942-7098","authenticated-orcid":false,"given":"Yoshiki","family":"Kawamura","sequence":"first","affiliation":[{"name":"Department of Pediatrics Fujita Health University School of Medicine Toyoake Japan"},{"name":"Deapartment of 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