{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:37:04Z","timestamp":1790217424797,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Torquetenovirus<\/jats:italic>\n                    load has been proposed as an immunomodulated biomarker of host immune status, yet its behavior\n                    <jats:italic>in situ<\/jats:italic>\n                    within HPV\u2010infected mucosa remains poorly defined. We conducted a retrospective cross\u2010sectional study of 220 patients undergoing HPV screening (181 cervical swabs, 39 anal brushings). HPV was genotyped with Allplex\u2122 HPV28, and TTV load was quantified by in\u2010house RT\u2010qPCR and expressed as log\n                    <jats:sub>10<\/jats:sub>\n                    copies per 20\u2009ng total DNA. Analyses included nonparametric group comparisons (Mann\u2010Whitney) and site\u2010stratified logistic regression to estimate the TTV\u2010HPV association. Genotype co\u2010occurrence was summarized by heatmaps and network analysis and formally tested with Fisher's exact test with Benjamini\u2010Hochberg FDR correction. TTV load was higher in HPV\u2010positive subjects (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), with a significant difference in BR and a trend in CS. In a binary logistic model, each 1\u2010log\n                    <jats:sub>10<\/jats:sub>\n                    increase in TTV was associated with a 60% increase in the odds of HPV positivity (OR\u2009=\u20091.60, 95% CI 1.07\u20132.39;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.022), with consistent results across CS and BR. TTV load increased with greater genotypic complexity in co\u2010infections (genotype richness) and was also higher in infections sustained exclusively by HR genotypes than by LR genotypes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). The co\u2010occurrence map and network analysis highlighted recurrent genotype combinations (e.g., 16\/53, 53\/68, 42\/53) and central nodes (16, 31, 51, 56, 68, 53).\n                    <jats:italic>In situ<\/jats:italic>\n                    quantification of TTV is associated with HPV positivity and with genotype complexity\/risk class, offering local predictive power. The co\u2010occurrence evidence remains exploratory but supports TTV as an indirect indicator of mucosal immunocompetence.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70850","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T13:50:13Z","timestamp":1771336213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["TTV Load Mirrors Local Immunity and Tracks HPV Positivity in the Anogenital Tract"],"prefix":"10.1002","volume":"98","author":[{"given":"Lilia","family":"Cinti","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology and Virology, Department of Molecular Medicine Sapienza University of Rome Rome Latium Italy"},{"name":"PhD of National Interest in Innovation in the diagnosis, prevention and treatment of infections at epidemic\u2010pandemic risk, Department of Medical Biotechnologies University of Siena Siena Tuscany Italy"},{"name":"University Hospital \u201cPoliclinico Umberto I\u201d Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Innocenza","family":"Palaia","sequence":"additional","affiliation":[{"name":"University Hospital \u201cPoliclinico Umberto I\u201d Rome Italy"},{"name":"Department of Gynecological, Obstetrical and Urological Sciences Sapienza University of Rome Rome Latium Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0633-360X","authenticated-orcid":false,"given":"Alessandra","family":"Pierangeli","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Virology, Department of Molecular Medicine Sapienza University of Rome Rome Latium Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"D'ettorre","sequence":"additional","affiliation":[{"name":"University Hospital \u201cPoliclinico Umberto I\u201d Rome Italy"},{"name":"Department of Public Health and Infectious Diseases Sapienza University of Rome Rome Latium Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenio Nelson","family":"Cavallari","sequence":"additional","affiliation":[{"name":"University Hospital \u201cPoliclinico Umberto I\u201d Rome Italy"},{"name":"Department of Public Health and Infectious Diseases Sapienza University of Rome Rome Latium Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2533-2939","authenticated-orcid":false,"given":"Guido","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Virology, Department of Molecular Medicine Sapienza University of Rome Rome Latium Italy"},{"name":"University Hospital \u201cPoliclinico Umberto I\u201d Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-3654","authenticated-orcid":false,"given":"Piergiorgio","family":"Roberto","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Virology, Department of Molecular Medicine Sapienza University of Rome Rome Latium Italy"},{"name":"University Hospital \u201cPoliclinico Umberto I\u201d Rome Italy"},{"name":"PhD National Program in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine University of Pavia Pavia Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2537"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021641"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/87.11.796"},{"key":"e_1_2_10_5_1","volume-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans","year":"2007"},{"issue":"6","key":"e_1_2_10_6_1","first-page":"25","article-title":"Genital Warts","volume":"5","author":"Yanofsky V. 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