{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:05:28Z","timestamp":1785524728857,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vaccinia virus (VACV) encodes a heterodimeric mRNA capping enzyme consisting of the catalytic large subunit D1 and the stimulatory small subunit D12. This is different from many other nucleocytoplasmic large DNA viruses, which encode the tripartite capping activities in one protein.\n                    <jats:italic>In vitro<\/jats:italic>\n                    studies indicate that while D12 lacks catalytic activity, it enhances D1's methyltransferase function. To define the functional requirement of D12 in VACV replication in infected cells, we generated a\n                    <jats:italic>D12L<\/jats:italic>\n                    (ORF encoding the D12 gene)\u2010deleted virus (v\u0394D12) using rabbit RK13 cells stably expressing D12. While v\u0394D12 replication was reduced and associated with impaired viral intermediate and late gene expression and altered plaque morphology, it remained permissive in RK13 cells even without D12 complementation. We further revealed that viral early gene expression was preserved in v\u0394D12\u2010infected cells, whereas viral DNA replication, intermediate and late gene expression was reduced. Interestingly, v\u0394D12 infection was unpermissive in monkey BS\u2010C\u20101 cells, with little intermediate and late gene expression. Furthermore, v\u0394D12 was unpermissive in rhesus macaque gastrointestinal organoids (enteroids), but remained permissive in human enteroids. These findings reveal differential requirements of D12 for VACV replication in host\u2010specific cells and enteroids.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70852","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:02:44Z","timestamp":1771588964000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vaccinia Virus Capping Enzyme Subunit D12 Is Differentially Required for Viral Replication in Different Cell Lines and Gastrointestinal Organoids"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1312-4258","authenticated-orcid":false,"given":"Yining","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University, College Station  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Pant","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University, College Station  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab R.","family":"Aboezz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University, College Station  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tibor","family":"Farkas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University, College Station  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8995-2654","authenticated-orcid":false,"given":"Zhilong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University, College Station  Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010199"},{"key":"e_1_2_9_3_1","first-page":"7","article-title":"Sedimentation of An Rna Polymerase Complex From Vaccinia Virus That Specifically Initiates and Terminates Transcription","volume":"7","author":"Broyles S. S.","year":"1987","journal-title":"Molecular and Cellular Biology"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74560-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(00)55003-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42020-0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)51108-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.6.3852-3856.1995"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2008.01.009"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.12.10020-10028.1998"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80236-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200532200"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42021-2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601912"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006602"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008926"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1099"},{"key":"e_1_2_9_18_1","first-page":"16","article-title":"Preparation of Cell Cultures and Vaccinia Virus Stocks","volume":"16","author":"Earl P. L.","year":"2001","journal-title":"Curr Protoc Mol Biol Chapter"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-876-4_13"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.5.2797-2808.1996"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00448-24"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5211"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70852","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.70852","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T17:41:08Z","timestamp":1785519668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/jmv.70852"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70852","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70852"}}