{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T10:51:07Z","timestamp":1775904667117,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007303","name":"University of Balamand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007303","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hepatitis E virus (HEV) is an emerging transfusion\u2010transmissible pathogen, yet epidemiological data from the Middle East remain limited, and differences in serological assay performance complicate interpretation of seroprevalence. Lebanon lacks national HEV surveillance data and has no blood donor screening policy. In this cross\u2010sectional national study, 3000 blood donors from all Lebanese governorates (July 2023\u2013August 2024) were screened for anti\u2010HEV IgG and IgM using two commercial platforms: Euroimmun ELISA and Roche Elecsys electrochemiluminescence immunoassay (ECLIA). This study represents the first large\u2010scale, peer\u2010reviewed evaluation of the Roche Elecsys Anti\u2010HEV assays in a national blood donor population. All samples that were positive or borderline by either serological assay were tested for HEV RNA using real\u2010time RT\u2010PCR (Altona RealStar\u00ae and Roche Cobas\u00ae). Anti\u2010HEV IgG seroprevalence differed significantly between assays (Euroimmun: 1.13% vs. Roche Elecsys: 1.93%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). IgM prevalence was 0.07% and 0.33%, respectively. Overall agreement between assays was high (IgG: 98.7%; IgM: 99.7%), although Cohen's \u03ba indicated limited concordance, with Roche Elecsys identifying additional low\u2010reactivity seropositive donors. Seropositivity varied across regions, with higher rates observed in South Lebanon (4.0%) and North Lebanon (3.1%). HEV RNA was not detected in any tested donor. HEV exposure among Lebanese blood donors is low but geographically distributed. Seroprevalence estimates were strongly assay\u2010dependent, indicating that HEV burden may be underestimated when lower\u2010sensitivity platforms are used. These findings highlight the importance of assay selection in HEV epidemiological studies and support consideration of selective HEV nucleic acid testing for high\u2010risk recipients to inform evidence\u2010based blood safety policy in Lebanon.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70857","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:27:38Z","timestamp":1772983658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence of Hepatitis E Virus Infection among Blood Donors in Lebanon: A National Comparative Evaluation of Two Serological Assays"],"prefix":"10.1002","volume":"98","author":[{"given":"Elissa","family":"Massihy","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences University of Balamand  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamima","family":"jisr","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department Makassed General Hospital  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Feghali","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Lebanese American University Gilbert and Rose Marie Chagoury School of Medicine  Byblos Lebanon"},{"name":"Lebanese Red Cross  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elie","family":"Khoury","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department Bhannes Hospital Center  Mount Lebanon Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walid","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department Makassed General Hospital  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rami","family":"Mahfouz","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine American University of Beirut Medical Center  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-5373","authenticated-orcid":false,"given":"Daniel","family":"Candotti","sequence":"additional","affiliation":[{"name":"Institut Mondor\u2010 INSERM U955 Team \u201cViruses, Hepatology, Cancer\u201d, and Department of Virology, Henri Mondor Hospital (AP\u2010HP) University Paris\u2010Est  Cr\u00e9teil France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wael","family":"Dimassi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Lebanese American University Gilbert and Rose Marie Chagoury School of Medicine  Byblos Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elias","family":"Abdo","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Balamand  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8889-4234","authenticated-orcid":false,"given":"Mira El","family":"Chaar","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences University of Balamand  Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Organization W. 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