{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T10:51:16Z","timestamp":1775904676277,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The study investigates the potential of involucrasins for COVID\u201019 prevention through molecular docking using AutoDock Vina and molecular dynamics (MD) simulations with Gromacs 2020. Docking results revealed that involucrasins exhibit strong binding affinity to the SARS\u2010CoV\u20102 Delta (B.1.617.2) and Omicron (BA.2 RBD) variants of SARS\u2010CoV\u20102, as well as the angiotensin\u2010converting enzyme 2 (ACE2) protein. MD simulations further confirmed their stable interactions with these targets. Additionally, bilayer interferometry (BLI) assays demonstrated that involucrasins effectively inhibited the binding between the receptor\u2010binding domain (RBD) of the Delta (B.1.617.2) and Omicron (BA.2 RBD) variants and ACE2, highlighting their potential as viral entry blockers. Further immunocytochemistry (ICC) and pseudo\u2010typed lentiviral particle assay indicated that the entry of the SARS\u2010CoV\u20102 Delta (B.1.617.2) and Omicron (BA.2 RBD) variants into host cells was impeded due to the inhibition of the binding of the spike (S) protein to ACE2 by involucrasins. ROS can contribute to the activation of the caspase\u20101 signaling pathway through their involvement in inflammasome activation. Involucrasins have an inhibitory effect on ROS generation in response to LPS\/ATP stimulation. Meantime, involucrasins reduce the level of caspase\u20101, IL\u20101\u03b2, and IL\u201033.<\/jats:p>","DOI":"10.1002\/jmv.70874","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:14:28Z","timestamp":1773836068000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Involucrasins Potentially Prevents SARS\u2010CoV\u20102 Infection via Inhibiting Caspase\u20101 Signaling in Several Variants"],"prefix":"10.1002","volume":"98","author":[{"given":"Mingming","family":"Wang","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization Yunnan University of Chinese Medicine Kunming Yunnan China"},{"name":"Institute of Traditional Chinese Medicine Pharmacology Shandong Academy of Chinese Medicine Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlian","family":"He","sequence":"additional","affiliation":[{"name":"Guangzhou National Laboratory Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization Yunnan University of Chinese Medicine Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanwan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy Shandong University of Traditional Chinese Medicine Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuya","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization Yunnan University of Chinese Medicine Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8741-4219","authenticated-orcid":false,"given":"Feng","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization Yunnan University of Chinese Medicine Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.01882-21"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.27526"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.66881"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.27643"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00630-8"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2665-2"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00884-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.052"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184331"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2025.108454"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111356"},{"key":"e_1_2_10_13_1","first-page":"565","article-title":"Forodesine and Riboprine Exhibit Strong Anti\u2010SARS\u2010CoV\u20102 Repurposing Potential: In Silico and In Vitro Studies","volume":"2","author":"Rabie A. 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