{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:59:28Z","timestamp":1781197168962,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mpox, caused by Monkeypox virus (MPXV), is associated with mucosal involvement and immune modulation that may influence viral coinfections. Merkel Cell Polyomavirus (MCPyV), a ubiquitous virus capable of lifelong persistence, was investigated in 66 Mpox patients enrolled at Lazzaro Spallanzani National Institute for Infectious Diseases (Rome, Italy; 2022\u20132025). Oropharyngeal and anal swabs collected during acute Mpox and, at 9\u2010month follow\u2010up, were analyzed by quantitative PCR, sequencing, transcript, and microRNA assays. MCPyV DNA was detected in 23\/66 (34.8%) individuals, with higher prevalence and load in anal (31.8%, 2.1\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    \u2009copies\/mL) than in oropharyngeal swabs (24.4%, 1.3\u2009\u00d7\u200910\n                    <jats:sup>2<\/jats:sup>\n                    \u2009copies\/mL;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). MCPyV persisted in 4\/10 (40%) oropharyngeal samples at follow\u2010up. No viral integration was observed, and full\u2010length Large Tumor Antigen was amplified in all samples. Transcript analysis revealed early and late genes; viral microRNAs were found in 3\/10 (30%) oropharyngeal and 5\/14 (35.7%) anal acute\u2010phase swabs, and persisted in 3\/4 (75%) MCPyV\u2010positive oropharyngeal samples at follow\u2010up. Among the 12 MCPyV\u2010positive people living with human immunodeficiency virus (HIV), MCPyV load was lower in oropharyngeal but higher in anal swabs compared to MCPyV\/MPXV cases. This study provides the first evidence of MCPyV detection in Mpox\u2010positive individuals and supports further investigation of its clinical relevance in coinfection settings.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70890","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T11:42:49Z","timestamp":1774611769000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and Molecular Profiling of Merkel Cell Polyomavirus in Patients With Monkeypox Virus Infection"],"prefix":"10.1002","volume":"98","author":[{"given":"Sara","family":"Passerini","sequence":"first","affiliation":[{"name":"Department of Public Health and Infectious Diseases Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Mariotti","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Laboratories of Biosecurity National Institute for Infectious Diseases Lazzaro Spallanzani \u2013 IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Messina","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Mazzotta","sequence":"additional","affiliation":[{"name":"Clinical Department National Institute for Infectious Diseases Lazzaro Spallanzani \u2013 IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-4243","authenticated-orcid":false,"given":"Giulia","family":"Matusali","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Laboratories of Biosecurity National Institute for Infectious Diseases Lazzaro Spallanzani \u2013 IRCCS Rome Italy"},{"name":"Department of Biology and Biotechnology \u201cCharles Darwin\u201d Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2121-4684","authenticated-orcid":false,"given":"Andrea","family":"Antinori","sequence":"additional","affiliation":[{"name":"Clinical Department National Institute for Infectious Diseases Lazzaro Spallanzani \u2013 IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Pietropaolo","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7489-5271","authenticated-orcid":false,"given":"Fabrizio","family":"Maggi","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Laboratories of Biosecurity National Institute for Infectious Diseases Lazzaro Spallanzani \u2013 IRCCS Rome 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