{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:46:09Z","timestamp":1787240769845,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Early, rapid, and sensitive detection of cytomegalovirus (CMV), Epstein\u2010Barr virus (EBV), human herpesvirus 6 (HHV\u20106), and herpes simplex virus Types 1\/2 (HSV\u20101\/2) is critical for improving clinical outcomes in pediatric hematopoietic stem cell transplantation (HSCT). A dual one\u2010tube recombinase\u2010aided PCR (DO\u2010RAP) assay was developed for the simultaneous detection of these viruses. The analytical sensitivity and specificity of the assay were evaluated and subsequently validated using 70 clinical whole blood samples. The DO\u2010RAP assay demonstrated limits of detection (LOD) of two copies\/reaction for CMV and HHV\u20106, 7 copies\/reaction for EBV, and three copies\/reaction for HSV\u20101\/2. The total detection time was 62\u2009min. In whole blood samples, DO\u2010RAP demonstrated 100% sensitivity relative to qPCR, and identified 37 additional positive cases among qPCR\u2010negative samples. DO\u2010RAP exhibited higher sensitivity than qPCR for monitoring viral status before HSCT, at 0\u201330 days post\u2010HSCT, and at 31\u201360 days post\u2010HSCT, enabling earlier detection of CMV, EBV, HHV\u20106, and HSV\u20101\/2 in recipients. During donor screening, DO\u2010RAP also identified one additional CMV\u2010positive and EBV\u2010positive case compared with qPCR. The DO\u2010RAP assay enables rapid and accurate detection of low\u2010level viral infections in whole blood samples from HSCT settings, providing a valuable tool for routine monitoring of HSCT\u2010associated viral infections.<\/jats:p>","DOI":"10.1002\/jmv.70905","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T11:01:26Z","timestamp":1775559686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sensitive and Rapid Dual One\u2010Tube Recombinase Aided PCR Assays for the Simultaneous Detection of Cytomegalovirus, Epstein\u2010Barr Virus, Human Herpesvirus 6, and Herpes Simplex Virus 1\/2 in Whole Blood From Children Undergoing Hematopoietic Stem Cell Transplantation"],"prefix":"10.1002","volume":"98","author":[{"given":"Li","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Diagnosis Hebei Medical University Shijiazhuang Hebei 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