{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T13:10:14Z","timestamp":1779369014823,"version":"3.53.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The study assessed the phenotypic susceptibility of non group\u2010M human immunodeficiency virus type 1 (HIV\u20101\/non\u2010M) clinical isolates to ibalizumab, the first\u2010in\u2010class long\u2010acting CD4\u2010directed post\u2010attachment inhibitor. Thirty isolates were tested: 1 HIV\u20101\/M (reference strain BRU.HXB2), 2 HIV\u20101\/N, 26 HIV\u20101\/O and 1 HIV\u20101\/P. A phenotypic in vitro assay was performed by pre\u2010incubating peripheral blood mononuclear cells with ibalizumab, (1\u201310\u2009000\u2009ng\/mL), then infected and incubated before quantifying viral load with RT\u2010PCR. IC\n                    <jats:sub>50<\/jats:sub>\n                    , maximum percent inhibition (MPI), and fold change (FC) were calculated. Potential\n                    <jats:italic>N<\/jats:italic>\n                    \u2010glycosylation sites (PNGS) in the V5 loop, V2 loop length and the side chain at position 375 were investigated. Ibalizumab inhibited 22 HIV\u20101\/O and 1 HIV\u20101\/N strains, with a median IC\n                    <jats:sub>50<\/jats:sub>\n                    of 0.0413 (range: 6.19E10\n                    <jats:sup>\u221211<\/jats:sup>\n                    ; 16.8)\u2009ng\/mL and a median MPI of 96.9 (range: 68.4; 99.3)%. The HIV\u20101\/P strain displayed potential resistance (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009&gt;\u200910\u2009000\u2009ng\/mL; MPI\u2009&lt;\u200925%). Interpretable results were not obtained for 4 HIV\u20101\/O and 1 HIV\u20101\/N strains under standardization criteria. PNGS numbers in the V5 loop ranged from 0 to 4 in tested isolates. Ibalizumab showed in vitro activity against all validated HIV\u20101\/O and HIV\u20101\/N isolates, whereas the HIV\u20101\/P strain exhibited potential natural resistance. No relationship was observed between PNGS number and susceptibility.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70911","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T10:31:11Z","timestamp":1775903471000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HIV\u20101 Non Group\u2010M Phenotypic Susceptibility to Ibalizumab"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5794-2131","authenticated-orcid":false,"given":"Lucie","family":"Poisson","sequence":"first","affiliation":[{"name":"Laboratoire de Virologie CHU de Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quentin Le","family":"Hingrat","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM UMR1137, IAME Paris France"},{"name":"AP\u2010HP Nord, D\u00e9partement de Vriologie CHU Bichat Claude\u2010Bernard Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Lermechain","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie CHU de Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Tombette","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie CHU de Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henri","family":"Jullien","sequence":"additional","affiliation":[{"name":"Theratechnologies Inc. Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Christophe","family":"Plantier","sequence":"additional","affiliation":[{"name":"DYNAMICURE UMR1311, CHU Rouen, D\u00e9partement de Virologie UnivRouen Normandie, Universit\u00e9 de Caen Normandie, INSERM, Normandie University Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8906-3416","authenticated-orcid":false,"given":"Elodie","family":"Alessandri\u2010Gradt","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie CHU de Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00012-13"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005029"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(92)90045-F"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000001791"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix1087"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.11.8893-8898.1997"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04201-14"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky271"},{"key":"e_1_2_9_10_1","unstructured":"Theratechnologies Inc. \u201cMonograph \u201d 2018."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000003218"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05669-11"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.03.004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.5.1095"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwh106"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e3182732746"},{"key":"e_1_2_9_17_1","article-title":"Assessment of Genotypic Patterns Associated With HIV\u20101 Sensitivity to Ibalizumab | AME","volume":"7","author":"Jullien H.","year":"2020","journal-title":"Reviews in Antiviral Therapy & Infectious Diseases"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03895-23"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1998.14.1281"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.10310-10319.1999"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02005-10"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70911","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.70911","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:47:51Z","timestamp":1779367671000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jmv.70911"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70911","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70911"}}