{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:24:11Z","timestamp":1789050251919,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515010148"],"award-info":[{"award-number":["2024A1515010148"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Zika virus (ZIKV), a single\u2010stranded positive\u2010sense RNA virus of the Flaviviridae family (\n                    <jats:italic>Flavivirus<\/jats:italic>\n                    genus), causes acute febrile illness and severe congenital anomalies. Serological cross\u2010reactivity with Dengue virus (DENV) and Chikungunya virus (CHIKV) complicates diagnosis, underscoring the urgency of developing specific point\u2010of\u2010care tests for early detection, outbreak mitigation, and reduced misdiagnosis risks. This study established and optimized an RPA\u2013CRISPR\/Cas12a assay for the rapid, visual, and highly specific detection of ZIKV. Primers and crRNAs targeting the highly conserved capsid (C) gene were designed, and the assay was systematically evaluated for its specificity, sensitivity, reproducibility, and clinical applicability. The RPA\u2013CRISPR\/Cas12a assay enabled naked\u2010eye detection under UV light within 35\u2009min. It demonstrated single\u2010copy sensitivity (1\u2009copy\/\u03bcL), no cross\u2010reactivity with DENV 1\u20134, CHIKV, or Japanese encephalitis virus (JEV), and 100% concordance with RT\u2010qPCR in clinical validation. Repeatability tests showed low variability (coefficient of variation (C.V.) &lt;\u200915%), confirming robust reproducibility. This instrument\u2010free platform integrates rapid visual detection, single\u2010copy sensitivity, high specificity, and field\u2010deployable features, making it particularly suitable for point\u2010of\u2010care testing (POCT) in resource\u2010limited settings. The developed assay provides critical support for early outbreak containment and prenatal screening in ZIKV\u2010endemic regions.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70917","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T07:11:29Z","timestamp":1776237089000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a Reverse Transcription Recombinase Polymerase Amplification CRISPR\/Cas12a Assay for Visual and Highly Specific Identification of Zika Virus"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4421-2375","authenticated-orcid":false,"given":"Xiao","family":"Cong","sequence":"first","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital Sun Yat\u2010Sen University Guangzhou Guangdong China"},{"name":"Institute of Pathogenic Microbiology Guangdong Provincial Center for Disease Control and 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