{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:00:59Z","timestamp":1791381659751,"version":"4.3.3"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Rotavirus (RV) is the leading cause of severe gastroenteritis in children and is responsible for significant morbidity and mortality in developing and low\u2010income countries. Hence, precise and prompt diagnosis of rotavirus infection is vital for treatment. The currently employed detection methods include rotavirus real\u2010time reverse transcriptase polymerase chain reaction (RT\u2010qPCR) and antigen\u2010capture enzyme\u2010linked immunosorbent assay (Ag\u2010ELISA) which have some known strengths and weaknesses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Rotaviruses are difficult to grow in various cell substrates; however, a few cell lines, including MA104, show evident virus growth. Hence, MA104 cell\u2010based immunocolorimetric assay (ICA) was developed, and its sensitivity and specificity were studied with the existing rotavirus detection methods that target the viral antigen or nucleic acid. A total of 84 fecal specimens obtained from acute gastroenteritis children were utilized, and the results of ICA were compared with commercial Ag\u2010ELISA and in\u2010house RT\u2010qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A cell culture\u2010based RV\u2010ICA was successfully standardized and validated with commercial Ag\u2010ELISA and in\u2010house RT\u2010qPCR. The sensitivity and specificity of ICA with commercial Ag\u2010ELISA were found to be 90.7% and 36.6%, respectively. The comparison of RT\u2010qPCR with RV\u2010ICA showed slightly lower sensitivity, that is, 84.3%, whereas increased specificity, that is, 57.1%. The positive predictive value of RV\u2010ICA with Ag\u2010ELISA and RT\u2010qPCR was 60% and 90.7%, respectively. It is well accepted that RT\u2010qPCR is a more sensitive and rapid assay for virus detection due to amplification and quantification of target nucleic acid. However, a cell culture\u2010based ICA has an added advantage over Ag\u2010ELISA and RT\u2010qPCR, due to added possibility of rotavirus isolation from positive fecal specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The standardized cell culture\u2010based RV\u2010ICA supports the detection of live rotaviruses from fecal specimens over the commercial Ag\u2010ELISA and RT\u2010qPCR, thus helps identify samples suitable for rotavirus isolation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.70943","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T14:00:23Z","timestamp":1777125623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of Cell Culture\u2010Based Immunocolorimetric Assay for Detection of Rotaviruses in Fecal Specimens Collected From the Acute Gastroenteritis Children"],"prefix":"10.1002","volume":"98","author":[{"given":"Neelakshi S.","family":"Kumbhar","sequence":"first","affiliation":[{"name":"ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya S.","family":"Suryawanshi","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabha","family":"Hela","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj G.","family":"Pandey","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3072-9313","authenticated-orcid":false,"given":"Mallika","family":"Lavania","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6157-9090","authenticated-orcid":false,"given":"Sunil R.","family":"Vaidya","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-022-01898-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1297269"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.20933"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf1159"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.Supplement_1.S65"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1679-45082016RB3582"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.046151-0"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.10.084"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-019-6406-0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-020-03622-3"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10040416"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2024.126551"},{"issue":"2","key":"e_1_2_10_14_1","first-page":"101","article-title":"Assay of Neutralizing Antibodies to Animal Rotavirus Strains and Human Rotavirus Serotype G8 by a Modified Method in the Residents of Pune, India","volume":"14","author":"Kelkar S. 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