{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:01:00Z","timestamp":1791381660372,"version":"4.3.3"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>To assess the preparedness and diagnostic capacity of Spanish clinical microbiology laboratories to detect the emerging monkeypox virus (MPXV) Clade Ib through a nationwide external quality assessment. A blinded 16\u2010sample panel (serum and DNA eluates) containing MPXV Clade Ib, Clade II, vaccinia, and negatives was distributed. Of 36 laboratories submitting results, 25 with clade\u2010level capability were included. Performance versus reference was adjudicated as full, partial, or discordant concordance; species\u2010level and clade\u2010specific metrics were estimated, and inter\u2010laboratory agreement summarized with simple and weighted kappa (Wilson 95% CIs; bootstrap for kappa CIs). Species\u2010level MPXV detection was consistently strong (mean sensitivity 85%, specificity 95%). Agreement was substantial (simple kappa 0.70; weighted 0.81). For Clade Ib, mean sensitivity was 50% (range: 0%\u2013100%) with high but variable specificity (100%). For clade II, mean sensitivity averaged 70% (25%\u2013100%) and specificity 98% (66.7%\u2013100%). Methodologies varied across centers: 68% used independent extraction plus commercial PCR, 20% mixed commercial extraction with in\u2010house PCR, and 12% integrated extraction\u2010PCR platforms. In conclusion, Spanish laboratories maintain good capacity for MPXV species\u2010level detection, but clade discrimination\u2014particularly for emerging Clade Ib\u2014remains heterogeneous and suboptimal across participating centers. Expanding QC panels to include new clades and updating\/validating assays should enhance national readiness for future transmission events.<\/jats:p>","DOI":"10.1002\/jmv.70948","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:50:45Z","timestamp":1777459845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Readiness Level of Spanish Labs to Detect Mpox Clade Ib: A Nationwide EQA"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-858X","authenticated-orcid":false,"given":"Adolfo","family":"De Salazar","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda Hospital Universitario San Cecilio Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria Ibs.Granada Granada Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anabel","family":"Negredo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Madrid Spain"},{"name":"Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III, Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Gal\u00e1n","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Del Peso","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III, Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Illescas","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda Hospital Universitario San Cecilio Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria Ibs.Granada Granada Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Mart\u00ednez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepci\u00f3n","family":"Gimeno","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital General de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7611-781X","authenticated-orcid":false,"given":"Federico","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda Hospital Universitario San Cecilio Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria Ibs.Granada Granada Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paz","family":"S\u00e1nchez\u2010Seco","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Madrid Spain"},{"name":"Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III, Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the QC Mpox Spain Collaborators","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"2023","volume-title":"Multi\u2010Country Outbreak of Mpox, External Situation Report 27\u201414","author":"World Health Organization","year":"2023"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)02075-X"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.29240"},{"key":"e_1_2_11_5_1","volume-title":"Mpox\u2014Democratic Republic of the Congo. Disease Outbreak News","author":"World Health Organization","year":"2024"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3108.241551"},{"key":"e_1_2_11_7_1","volume-title":"2022\u201325 Mpox (Monkeypox) Outbreak: Global Trends [Dashboard]","author":"World Health Organization","year":"2025"},{"key":"e_1_2_11_8_1","unstructured":"European Centre for Disease Prevention and Control (ECDC).Local Transmission of Clade 1b Mpox Cases Detected in EU\/EEA\u2014ECDC Urges Renewed Vigilance. 24 Oct2025.https:\/\/www.ecdc.europa.eu\/en\/news-events\/local-transmission-clade-1b-mpox-cases-detected-eueea-ecdc-urges-renewed-vigilance."},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31224-2"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01044-8"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(23)00198-5"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2024.29.32.2400486"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2024.105741"},{"key":"e_1_2_11_15_1","volume-title":"Risk Assessment for the EU\/EEA of the Mpox Epidemic Caused by Monkeypox Virus Clade I in Africa\u201416 August 2024","author":"European Centre for Disease Prevention and Control (ECDC)","year":"2024"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47518-8"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c05007"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202404320"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70948","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.70948","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70948","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:48:30Z","timestamp":1791380910000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/jmv.70948"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70948","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]},"assertion":[{"value":"2025-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70948"}}