{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:00:56Z","timestamp":1791381656869,"version":"4.3.3"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diagnosing HIV\u20101 infection as early as the primary infection is crucial. In this context, we performed a comparative evaluation and agreement analysis of the newly proposed serological assay for the combined detection of total antibodies against HIV and P24 antigen (HIV VIDAS\u00ae DUO AG\/AB, bioM\u00e9rieux\u00ae), with the previously available HIV VIDAS\u00ae DUO Ultra assay in the well characterized acute\u2010HIV\u2010infection multicentric ANRS\u2010CO\u201006\u2010PRIMO Cohort in classification of Fiebig stages combined with confirmatory testing. Samples collected on the day of enrollment in the cohort, between April 2024 and June 2025, were analyzed with both serological assays. We also performed a recency infection assay, an HIV\u20101 westernblotting and the measurement of HIV\u2010RNA. Acute infection was classified according to Fiebig stages and compared between both assays. All samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200977) were positive with both assays. One major discordance was noted, with a positive antigen and a negative antibody reaction with DUO AG\/AB, with an opposite configuration with DUO Ultra. A high proportion of not determinable signals (26% to 68%) was noted with both assays, prompting a difficulty in classifying acute infection stages in the absence of confirmatory assays. The high rate of not determinable signals especially with P24 antigen with both assays demonstrated in our study, could prompt a difficulty in acute infection classification according to Fiebig.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70953","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T14:00:08Z","timestamp":1777903208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Evaluation of the New HIV VIDAS\u00ae DUO AG\/AB Assay With DUO Ultra on Well Characterized Participants From the HIV\u20101 Acute Infection French ANRS PRIMO Cohort"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2094-0492","authenticated-orcid":false,"given":"Gilbert","family":"Mchantaf","sequence":"first","affiliation":[{"name":"CHU d'Orl\u00e9ans, Virologie Orl\u00e9ans France"},{"name":"Universit\u00e9 d'Orl\u00e9ans, LI2RSO Orl\u00e9ans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Maia","sequence":"additional","affiliation":[{"name":"Universit\u00e9 d'Orl\u00e9ans, LI2RSO Orl\u00e9ans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Andriantsoanirina","sequence":"additional","affiliation":[{"name":"AP\u2010HP, H\u00f4pital Bic\u00eatre, Sant\u00e9 Publique Paris France"},{"name":"INSERM CESP U1018, Universit\u00e9 Paris Saclay Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oc\u00e9ane","family":"Dehan","sequence":"additional","affiliation":[{"name":"CHU d'Orl\u00e9ans, Virologie Orl\u00e9ans France"},{"name":"Universit\u00e9 d'Orl\u00e9ans, LI2RSO Orl\u00e9ans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9686-9194","authenticated-orcid":false,"given":"Karl","family":"Stefic","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, INSERM U1259, CHRU de Tours Tours France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2264-4822","authenticated-orcid":false,"given":"Laurent","family":"Hocqueloux","sequence":"additional","affiliation":[{"name":"Universit\u00e9 d'Orl\u00e9ans, LI2RSO Orl\u00e9ans France"},{"name":"CHU d'Orl\u00e9ans, service des maladies infectieuses et tropicales Orl\u00e9ans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Robineau","sequence":"additional","affiliation":[{"name":"H\u00f4pital Guy Chatiliez Tourcoing France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Makinson","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department &amp; INSERMU1175 Montpellier University Hospital Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Goujard","sequence":"additional","affiliation":[{"name":"INSERM CESP U1018, Universit\u00e9 Paris Saclay Paris France"},{"name":"Service de M\u00e9decine Interne et d'Immunologie Clinique, H\u00f4pital Bic\u00eatre, APHP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Meyer","sequence":"additional","affiliation":[{"name":"AP\u2010HP, H\u00f4pital Bic\u00eatre, Sant\u00e9 Publique Paris France"},{"name":"INSERM CESP U1018, Universit\u00e9 Paris Saclay Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Avettand\u2010Fenoel","sequence":"additional","affiliation":[{"name":"CHU d'Orl\u00e9ans, Virologie Orl\u00e9ans France"},{"name":"Universit\u00e9 d'Orl\u00e9ans, LI2RSO Orl\u00e9ans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"ANRS PRIMO cohort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks533"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004543"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033948"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03570-23"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aav3491"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.06.019"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jve.2023.100357"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/s2666-5247(21)00010-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv084"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1972"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200309050-00005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4441-4447.2005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ171"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-44389-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1931"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003211"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/qad.0b013e32833b61ba"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy479"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0000000000000891"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56116-1"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70953","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.70953","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70953","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:48:08Z","timestamp":1791380888000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70953"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/jmv.70953"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70953","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70953"}}