{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:00:56Z","timestamp":1791381656936,"version":"4.3.3"},"reference-count":82,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["404138\/2012\u20108"],"award-info":[{"award-number":["404138\/2012\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dengue is classified into mild dengue, which is treated at outpatient clinics, dengue with warning signs (WS+), which requires hospitalization, and severe dengue, which can be fatal. It is assumed that severe manifestations result from uncontrolled immune activation. Regulatory T cells (Tregs) are crucial for controlling inflammation in various diseases, but are poorly studied in dengue. This study aimed to investigate the Tregs phenotype in different clinical forms of dengue infection. Plasma and peripheral blood mononuclear cells (PBMCs) were collected from healthy donors and dengue patients. PBMCs were cultured in the presence of peptide libraries encoding the envelope (ENV) and NS3 viral proteins of DENV1. WS+ dengue patients presented higher plasma IL\u201010 levels than mild dengue during the febrile phase. In contrast, mild cases displayed a balanced proinflammatory and regulatory cytokine profile with high levels of IFN\u03b3, TNF, and IL\u201010 in the plasma compared to WS+ group during the critical phase. Mild dengue patients also showed higher frequencies of Tregs with increased expression of GITR (Glucocorticoid\u2010Induced TNFR\u2010Related) and CD200 when compared with WS+ dengue, both in critical and convalescent phases. In contrast, frequencies of PD1+ Tregs, which can indicate Tregs dysfunction, were increased in WS+ dengue subjects. Importantly, DENV\u2010specific GITR+ Tregs were a source of IL\u201010 in mild dengue, but this population was absent from WS+ patients. While WS+ dengue patients presented altered regulatory phenotypes by Tregs, the presence of IL\u201010 by DENV\u2010specific GITR+ Tregs and CD200+ Tregs was associated with mild presentation of dengue, suggesting that these regulatory mechanisms play an important role in limiting dengue immunopathology.<\/jats:p>","DOI":"10.1002\/jmv.70956","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T10:42:59Z","timestamp":1778150579000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DENV\u2010Specific IL\u201010\u2010Producing Regulatory T Cells Are Associated With Mild Clinical Outcomes in Dengue"],"prefix":"10.1002","volume":"98","author":[{"given":"Marcela Helena","family":"Gon\u00e7alves\u2010Pereira","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology Federal University of Minas Gerais Belo Horizonte Minas Gerais 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