{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:07:03Z","timestamp":1784196423855,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vaginal self\u2010sampling is increasingly integrated into cervical cancer screening programs, requiring evidence of accurate HPV testing on self\u2010collected samples. This study evaluated the clinical accuracy of Allplex HPV HR Detection (Allplex) within VALHUDES, a framework established to assess the clinical performance of HPV assays on self\u2010collected vaginal versus clinician\u2010collected cervical samples. A total of 523 women, recruited at Belgian colposcopy clinics, provided vaginal self\u2010samples using either the Evalyn Brush or Qvintip whereas gynaecologists subsequently collected cervical samples with a Cervex Brush. Both vaginal and cervical specimens were resuspended in 20\u2009mL of ThinPrep medium. Paired specimens from 499 women were tested with Allplex. The relative clinical sensitivity of vaginal self\u2010samples collected with Evalyn Brush and Qvintip for high\u2010grade cervical intraepithelial neoplasia (CIN2+) versus clinician\u2010collected cervical samples was 0.96 (95% CI: 0.91\u20131.02) and 0.91 (95% CI: 0.82\u20131.01), respectively. Both self\u2010sampling devices demonstrated higher specificity for &lt; CIN2 compared to clinician\u2010collected cervical samples, with relative specificity of 1.13 (95% CI: 1.02\u20131.25) on vaginal self\u2010samples collected with Evalyn Brush and 1.09 (95% CI: 0.99\u20131.19) for those collected with Qvintip. Median Ct\u2010values for overall hrHPV, HPV16, 31, 45, 52, 58 and human\n                    <jats:italic>Beta\u2010globin<\/jats:italic>\n                    gene were significantly lower in clinician\u2010collected cervical compared to vaginal self\u2010samples. In conclusion, Allplex demonstrated comparable clinical sensitivity and higher specificity on vaginal self\u2010 versus clinician\u2010collected cervical samples, without the need for cut\u2010off optimization.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70982","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:42:13Z","timestamp":1781156533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Accuracy of Allplex HPV HR Detection Full Genotyping Assay on Cervical Samples Compared to Vaginal Self\u2010Samples"],"prefix":"10.1002","volume":"98","author":[{"given":"Ardashel","family":"Latsuzbaia","sequence":"first","affiliation":[{"name":"Unit of Cancer Epidemiology, Belgian Cancer Centre, Sciensano Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Severien Van","family":"Keer","sequence":"additional","affiliation":[{"name":"Centre for the Evaluation of Vaccination (CEV), Vaccine &amp; Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences University of Antwerp, Edegem Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davy Vanden","family":"Broeck","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pathology AML Sonic Healthcare Antwerp Belgium"},{"name":"National Reference Centre for HPV Brussels Belgium"},{"name":"AMBIOR, Laboratory for Cell Biology &amp; Histology University of Antwerp Antwerp Belgium"},{"name":"International Centre for Reproductive Health Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Weyers","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Ghent University Hospital Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Donders","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The General Regional Hospital Heilig Hart Tienen Belgium"},{"name":"Femicare vzw, Clinical Research for Women Tienen Belgium"},{"name":"Department of Obstetrics and Gynaecology University Hospital Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe De","family":"Sutter","sequence":"additional","affiliation":[{"name":"Department Gynaecology\u2010Oncology UZ Brussel \u2013 VUB Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6618-045X","authenticated-orcid":false,"given":"Wiebren","family":"Tjalma","sequence":"additional","affiliation":[{"name":"Multidisciplinary Breast Clinic, Unit Gynaecologic Oncology, Department of Obstetrics and Gynaecology Antwerp University Hospital (UZA) Edegem Belgium"},{"name":"Molecular Imaging, Pathology, Radiotherapy, Oncology (MIPRO), Faculty of Medicine and Health Sciences University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Doyen","sequence":"additional","affiliation":[{"name":"Department Gynaecology\u2010Obstetrics University Hospital Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Vorsters","sequence":"additional","affiliation":[{"name":"International Centre for Reproductive Health Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Arbyn","sequence":"additional","affiliation":[{"name":"Unit of Cancer Epidemiology, Belgian Cancer Centre, Sciensano Brussels Belgium"},{"name":"Department of Human Structure and Repair, Faculty of Medicine and Health Sciences University Ghent Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66800-5_1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25065"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(22)00241-8"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2020.106399"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.k4823"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2021.106900"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/ijgc-2020-001285"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34434"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2144\/btn-2022-0084"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-22-1041"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(13)70570-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2018.08.006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2021.06.010"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01631-22"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-22-0757"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2023.06.004"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2023.09.008"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2022.105271"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2025.02.004"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.70273"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-012799"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2023.105638"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00332-24"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep07439"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24010"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25144"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2024.105737"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.70079"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02872-23"},{"key":"e_1_2_10_32_1","first-page":"1","article-title":"Cervical Cancer Screening","volume":"18","author":"IARC","year":"2022","journal-title":"IARC Handb Cancer Prev"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2021.104756"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12905-023-02691-8"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.70482"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.70982","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:32:34Z","timestamp":1784194354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/jmv.70982"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70982","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70982"}}