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This study discovers TXD\u2010198 as a potent inhibitor of SARS\u2010CoV\u20102 infection. Through a transcription\u2010 and replication\u2010competent virus\u2010like particle (trVLP) system, it's shown that TXD\u2010198 efficiently suppresses viral activity with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 1.04\u2009\u03bcM and a high selectivity index of 42.55. Validation in two distinct pseudoviral models\u2010the replication\u2010competent rVSV\u2010Venus\u2010VSV\u2206G\u2010SARS2\u2010S\u220621 system and SARS\u2010CoV\u20102 spike\u2010pseudotyped lentiviral system (PSVs) further confirmed TXD\u2010198's antiviral ability. Transcriptomic analysis reveals that TXD\u2010198 exerts antiviral effects through a dual mechanism of direct viral inhibition and anti\u2010inflammatory activity. Notably, TXD\u2010198 significantly upregulates the expression of the ANO1 gene, and knocking down ANO1 reverses the antiviral effect of TXD\u2010198, indicating ANO1's crucial role in TXD\u2010198's antiviral action. These findings suggest TXD\u2010198 is a promising therapeutic candidate for COVID\u201019 treatment related to ANO1, calling for further exploration of its clinical potential.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71004","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T08:27:04Z","timestamp":1781080024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TXD\u2010198 Exhibits Inhibitory Activity Against SARS\u2010CoV\u20102 by Upregulating ANO1"],"prefix":"10.1002","volume":"98","author":[{"given":"Ruilin","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences Southern Medical University  Guangzhou P. R. 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