{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:39:06Z","timestamp":1792463946694,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Human adenovirus<\/jats:italic>\n                    (HAdV) is a leading cause of acute respiratory tract infections (ARTIs) in children. The high prevalence of HAdV serotypes 3 and 7 in regions such as China presents a significant public health challenge. Here, we propose a one\u2010pot assay that integrates multiple cross displacement amplification (MCDA) with CRISPR\u2010Cas12b for the detection of HAdV\u20103 and HAdV\u20107, termed HAdV\u2010MCDA\u2010One. In this system, MCDA provides exponential target amplification, while the collateral cleavage activity of Cas12b enables secondary signal amplification. The entire reaction is performed isothermally at 60\u00b0C in a single tube, providing a fluorescent readout within 50\u2009min, making the assay suitable for point\u2010of\u2010care testing (POCT). Leveraging the single\u2010base recognition capability of CRISPR\u2010Cas12b, the assay demonstrates high specificity, with no cross\u2010reactivity observed against the other 13 identified pathogens. The limit of detection was determined to be 1.59 copies per reaction using target plasmids. Moreover, when evaluated with 96 clinical pharyngeal swabs, the assay showed 100% concordance with quantitative PCR (qPCR), confirming its clinical reliability. These results demonstrate HAdV\u2010MCDA\u2010One as a rapid and robust tool for HAdV\u20103 and HAdV\u20107 detection, with significant potential for clinical diagnosis and public health surveillance.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71032","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T11:06:59Z","timestamp":1783163219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A One\u2010Pot CRISPR\u2010Cas12b Assay for Rapid Detection of Human Adenovirus Serotypes 3 and 7"],"prefix":"10.1002","volume":"98","author":[{"given":"Linglong","family":"Wan","sequence":"first","affiliation":[{"name":"Experimental Research Center, Capital Center for Children's Health Capital Medical University, Capital Institute of Pediatrics Beijing 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for Children's Health Capital Medical University, Capital Institute of Pediatrics Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Fu","sequence":"additional","affiliation":[{"name":"Experimental Research Center, Capital Center for Children's Health Capital Medical University, Capital Institute of Pediatrics Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Experimental Research Center, Capital Center for Children's Health Capital Medical University, Capital Institute of Pediatrics Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Jia","sequence":"additional","affiliation":[{"name":"Experimental Research Center, Capital Center for Children's Health Capital Medical University, Capital Institute of Pediatrics Beijing 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