{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:11:46Z","timestamp":1791515506313,"version":"4.3.4"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Early diagnosis of Hepatitis C virus (HCV) infection is a cornerstone of elimination strategies. Fourth\u2010generation antigen\/antibody combination assays, such as Elecsys HCV Duo, promise to bridge the diagnostic gap left by antibody\u2010only testing. In this real\u2010world study, we assessed Elecsys HCV Duo performance using seroconversion panels and routine clinical samples, focusing on sensitivity, specificity, and positive predictive value (PPV) for active HCV detection, including challenging scenarios with low viral loads and HIV co\u2010infections. The assay shortened the serological window by up to 203 days. When both antigen and antibody were detected, PPV reached 100%, enabling confident diagnosis without immediate RNA confirmation. The antigen component alone achieved a PPV of 97.6%, even in a low\u2010prevalence setting (0.33%), better than previously reported assays. However, sensitivity for low viral loads (\u2264\u20095 log\n                    <jats:sub>10<\/jats:sub>\n                    IU\/mL) was limited, with 95.5% of such cases missed. Elecsys HCV Duo offers a powerful tool for earlier detection and streamlined care, particularly in high\u2010risk and resource\u2010limited contexts. Yet, confirmatory RNA testing remains essential for low viral load cases. Broader validation is needed to confirm its utility in diverse populations, reinfection surveillance, and cost\u2010effective screening strategies.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71050","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T12:15:29Z","timestamp":1783167329000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive Evaluation of the Elecsys HCV Duo Immunoassay for Active Hepatitis C Detection: Insights From the DEPCDUO Study"],"prefix":"10.1002","volume":"98","author":[{"given":"Paul\u2010Elie","family":"Gosset","sequence":"first","affiliation":[{"name":"Department of Virology Hospices Civils de Lyon Lyon France"},{"name":"Universit\u00e9 de Lyon, Lyon 1 Universit\u00e9 Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia","family":"Margueron\u2010Sentis","sequence":"additional","affiliation":[{"name":"Department of Virology Hospices Civils de Lyon Lyon France"},{"name":"Lyon Hepatology Institute, IHU EVEREST Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1981-7155","authenticated-orcid":false,"given":"Christophe","family":"Rami\u00e8re","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, Lyon 1 Universit\u00e9 Lyon France"},{"name":"Lyon Hepatology Institute, IHU EVEREST Lyon France"},{"name":"Department of Virology, INSERM Unit 1111, CIRI Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Zoulim","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, Lyon 1 Universit\u00e9 Lyon France"},{"name":"Lyon Hepatology Institute, IHU EVEREST Lyon France"},{"name":"Department of Hepatology, INSERM Unit 1350 PathLiv Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0691-8480","authenticated-orcid":false,"given":"Caroline","family":"Scholt\u00e8s","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, Lyon 1 Universit\u00e9 Lyon France"},{"name":"Lyon Hepatology Institute, IHU EVEREST Lyon France"},{"name":"Department of Virology, INSERM Unit 1350 PathLiv Hospices Civils de Lyon Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,4]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"World Health Organization \u201cGlobal Hepatitis Report 2024: Action for Access in Low\u2010 and Middle\u2010Income Countries [Internet] \u201d accessed December 18 2024 https:\/\/www.who.int\/publications\/i\/item\/9789240091672."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.14218\/JCTH.2017.00067"},{"key":"e_1_2_11_4_1","unstructured":"HAS \u201cRecommandations de Prise en Charge des Personnes Infect\u00e9es par le Virus de l'H\u00e9patite C \u201d2024."},{"key":"e_1_2_11_5_1","unstructured":"HAS \u201cStrat\u00e9gies de D\u00e9pistage Biologique des H\u00e9patites Virales B et C 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