{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:27:32Z","timestamp":1787668052885,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Respiratory tract infections (RTIs) pose a major diagnostic challenge, complicated by multiple microbial etiologies. Conventional low\u2010throughput methods may underestimate the prevalence and impact of co\u2010infections. This study aimed to evaluate a novel super\u2010multiplex microfluidic qPCR system for comprehensive 49 target pathogens screening and co\u2010infection analysis in patients with influenza\u2010like illness (ILI). Throat swab samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009288) were collected from febrile patients in Beijing during the spring of 2025. All samples were tested using the super\u2010multiplex microfluidic qPCR chip, with a subset (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200996) validated by conventional qPCR to determine analytical sensitivity, specificity, and Cohen's kappa. The assay demonstrated an overall pathogen detection rate of 66.32%. Influenza A virus (IAV) was the most prevalent pathogen, identified in 50.00% of all samples and constituting 70.69% of single infections, followed by SARS\u2010CoV\u20102 (14.24%) and Epstein\u2013Barr virus (EBV, 11.81%). Co\u2010infections were frequent (39.27% of positive cases), predominantly dual (23.04%) and triple (10.47%) infections. Notably, IAV was implicated in 84.0% of co\u2010infections. The microfluidic qPCR showed high concordance with conventional qPCR (sensitivity 91.07%, specificity 92.50%,\n                    <jats:italic>\u03ba<\/jats:italic>\n                    \u2009=\u20090.83, indicating almost perfect agreement). This study reveals a complex respiratory pathogen landscape dominated by IAV and marked by frequent polymicrobial co\u2010infections. The super\u2010multiplex microfluidic qPCR chip is a reliable, high\u2010throughput platform that effectively addresses the need for improved detection of co\u2010infections, offering valuable insights for both clinical decision\u2010making and public health surveillance.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71072","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:26:57Z","timestamp":1785137217000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of a Super\u2010Multiplex Microfluidic qPCR System for Detection of Respiratory Pathogens in Patients With Influenza\u2010Like Illness"],"prefix":"10.1002","volume":"98","author":[{"given":"Yuan","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences Beijing 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