{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:29:17Z","timestamp":1787671757101,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Respiratory viral infections, particularly Influenza A and SARS\u2010CoV\u20102, remain major public health concerns in endemic settings such as Pakistan, where high influenza\u2010like illness burden and limited genomic data underscore the need for surveillance to guide vaccine strategies. This study assessed respiratory virus circulation and genetic diversity among ILI cases at a secondary\u2010care hospital in Islamabad during the 2025\u20132026 winter season. Among 170 qRT\u2010PCR\u2010tested cases, Influenza A was detected in 24.1%, SARS\u2010CoV\u20102 in 1.17%, and Influenza B was not detected. Influenza A positivity was slightly higher in females (25.0%), with highest burden in individuals aged 11\u201320 years. Whole\u2010genome sequencing of 21 Influenza A(H3N2) isolates revealed co\u2010circulation of subclade K (52.4%), J.2.2 (28.6%), and J.2 (9.5%). Phylogenetic analysis showed subclade K clustering with recent isolates from North America, Oceania, Europe, and WHO\u2010recommended 2026\u20132027 Northern Hemisphere H3N2 vaccine strains, whereas J.2 and J.2.2 aligned with earlier vaccine\u2010like strains. NA analysis showed predominance of clade B.4.2.2 associated with K\u2010lineage and relatedness to recent North American strains. Subclade K exhibited conserved HA substitutions (K2N, S144N, N158D, I160K, Q173R, T328A) and HA2 S49N with limited variation. No antiviral resistance mutations were detected. Overall, findings demonstrate predominance and genetic stability of H3N2 subclade K and support continuous genomic surveillance for vaccine strain selection and influenza preparedness.<\/jats:p>","DOI":"10.1002\/jmv.71075","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T18:40:14Z","timestamp":1784745614000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Surveillance and Genomic Characterization of Influenza A(H3N2) Viruses From a Secondary\u2010Care Surveillance Site in Pakistan During the 2025\u20132026 Winter Season"],"prefix":"10.1002","volume":"98","author":[{"given":"Aamir","family":"Javed","sequence":"first","affiliation":[{"name":"Department of Biotechnology Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamal","family":"Hayat","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Adnan","family":"Haider","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunera","family":"Jamal","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia","family":"Hakim","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qasim","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mir Muhammad Hassan","family":"Bullo","sequence":"additional","affiliation":[{"name":"Federal General Hospital, Chak Shahzad, M4PQ+MMC General Wards Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Tahir","sequence":"additional","affiliation":[{"name":"Federal General Hospital, Chak Shahzad, M4PQ+MMC General Wards Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Salman","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusra","family":"Javed","sequence":"additional","affiliation":[{"name":"Health Services Academy, Prime Minister's National Health Complex Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5789-4215","authenticated-orcid":false,"given":"Yasir","family":"Waheed","sequence":"additional","affiliation":[{"name":"Irfan Suat Gunsel Operational Research Institute Near East University Istanbul Turkey"},{"name":"Sz\u00e9chenyi Istv\u00e1n University Gy\u0151r Hungary"},{"name":"VIZJA University Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massab","family":"Umair","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Health Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2024.06.004"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003657"},{"key":"e_1_2_11_4_1","first-page":"1","article-title":"Influenza A Virus","volume":"1","author":"Suarez D. 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