{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:32:06Z","timestamp":1791250326370,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In 2023 and 2024, Esp\u00edrito Santo state, Brazil, experienced its largest dengue epidemic in 30 years, with record numbers of cases and deaths. This study aimed to characterize confirmed dengue cases and the dengue virus (DENV) during the epidemic and pre\u2010epidemic periods. A population\u2010based descriptive study integrated with cross\u2010sectional genomic sampling was conducted using data from the eSUS Health Surveillance System (2020\u20132024) and molecular analysis of serum samples (2023\u20132024). Whole 26 DENV genomes were obtained and combined with public data for phylogenetic reconstruction. In 2023, 127\u2009070 dengue cases were reported; 2.8% showed warning signs or severe dengue, 2.6% required hospitalization, and 99 cases resulted in death. In 2024, 131\u2009170 cases were reported, with 2.0% showing warning signs or severe dengue, 2.3% requiring hospitalization, and 42 fatalities. Four lineages were detected: DENV\u20101 genotype V lineages 1V_A and 1V_E.1, DENV\u20102 genotype II lineage 2II_F.1.1.2, and genotype III lineage 2III_C.1.1. These lineages were closely related to viruses from other regions in Brazil and South America. Our findings highlight the complex dynamics of DENV in Esp\u00edrito Santo state and hypothesize that the multiple introduced DENV lineages and genetic diversity may have contributed to the magnitude and sustained transmission observed over consecutive years.<\/jats:p>","DOI":"10.1002\/jmv.71078","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:41:44Z","timestamp":1785415304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic and Epidemiological Characterization of DENV\u20101 and DENV\u20102 Co\u2010Circulation During the 2023\u20132024 Dengue Epidemic in Esp\u00edrito Santo, Brazil"],"prefix":"10.1002","volume":"98","author":[{"given":"Nicolli","family":"Ribeiro de Jesus","sequence":"first","affiliation":[{"name":"Post\u2010Graduate Program in Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caio","family":"Santos de Souza","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariene","family":"R. Amorim","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Paulo","family":"Cola","sequence":"additional","affiliation":[{"name":"Special Epidemiological Surveillance Unit Health Department of Esp\u00edrito Santo State Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3670-2690","authenticated-orcid":false,"given":"Raquel","family":"Gomes Catozo","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Luiz","family":"M. Guedes","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2165-5073","authenticated-orcid":false,"given":"Bruna","family":"Caetano Pimenta","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Program in Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4461-0045","authenticated-orcid":false,"given":"Julia Sthefany","family":"Nunes Zordan","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Program in Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmim","family":"Barcellos Madeira Rosa","sequence":"additional","affiliation":[{"name":"School of Biology Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1920-8915","authenticated-orcid":false,"given":"Henrique","family":"Tamanini Silva Moschen","sequence":"additional","affiliation":[{"name":"School of Biology Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Souza Areias","sequence":"additional","affiliation":[{"name":"Epidemiological Surveillance Service Health Department of Vit\u00f3ria Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane","family":"Comerio","sequence":"additional","affiliation":[{"name":"Epidemiological Surveillance Service Health Department of Vit\u00f3ria Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela","family":"Valim Sarmento","sequence":"additional","affiliation":[{"name":"Nucleus of Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Miranda Fardin","sequence":"additional","affiliation":[{"name":"Nucleus of Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Claudio","family":"de Oliviera Gomes","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Program in Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"},{"name":"Nucleus of Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiven","family":"Kumar","sequence":"additional","affiliation":[{"name":"Program in Emerging Infectious Diseases Duke\u2010NUS Medical School Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eng Eong","family":"Ooi","sequence":"additional","affiliation":[{"name":"Program in Emerging Infectious Diseases Duke\u2010NUS Medical School Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Malta Romano","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Creuza Rachel","family":"Vicente","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Program in Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"},{"name":"Nucleus of Infectious Diseases Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/25310429.2024.2448364"},{"key":"e_1_2_12_3_1","unstructured":"Minist\u00e9rio da Sa\u00fade Atualiza\u00e7\u00e3o de Casos de Arboviroses [Ministry of Health Update on Arbovirus Cases](Brasil Minist\u00e9rio da Sa\u00fade 2024) https:\/\/www.gov.br\/saude\/pt-br\/assuntos\/saude-de-a-a-z\/a\/aedes-aegypti\/monitoramento-das-arboviroses."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0113-2024"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0012397"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12080829"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002834"},{"key":"e_1_2_12_8_1","unstructured":"Minist\u00e9rio da Sa\u00fade Dengue: diagn\u00f3stico e manejo cl\u00ednico \u2010 adulto e crian\u00e7a [Ministry of Health Dengue: Clinical Diagnosis and Management \u2013 Adults and Children] 6th ed. (Minist\u00e9rio da Sa\u00fade 2024)."},{"key":"e_1_2_12_9_1","unstructured":"Secretaria de Estado da Sa\u00fade do Esp\u00edrito Santo Monitoramento da Dengue no Esp\u00edrito Santo [Esp\u00edrito Santo State Department of Health Dengue Monitoring in Esp\u00edrito Santo](Secretaria de Estado da Sa\u00fade do Esp\u00edrito Santo 2024) https:\/\/app.powerbi.com\/view?r=eyJrIjoiNDdlY2E2MWMtZTY2My00ZmM2LWI4YTctOTljYTI3NWM4NmZiIiwidCI6IjhiMjA4ZmViLTIyMTYtNDQ1Zi1iZmQxLTk1MjU4ZDlkMjExMSJ9."},{"key":"e_1_2_12_10_1","unstructured":"Governo do Estado do Esp\u00edrito Santo Plano de conting\u00eancia estadual das arboviroses dengue Zika e Chikungunya 2021\u20112023 [Government of the State of Esp\u00edrito Santo State Contingency Plan for Arboviruses: Dengue Zika and Chikungunya 2021\u20132023](Secretaria da Sa\u00fade 2024)."},{"key":"e_1_2_12_11_1","unstructured":"Funda\u00e7\u00e3o Oswaldo Cruz \u201cDashboard Rede Gen\u00f4mica Fiocruz \u2010 Dengue [Fiocruz Genomic Network Dashboard \u2013 Dengue] \u201dhttps:\/\/www.genomahcov.fiocruz.br\/dashboard-denv-pt\/."},{"key":"e_1_2_12_12_1","unstructured":"World Health Organization Dengue \u2013 Global Situation(WHO 2024) https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2024-DON518."},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2025.05.007"},{"key":"e_1_2_12_14_1","unstructured":"Instituto Brasileiro de Geografia e Estat\u00edstica (IBGE) Cidades: Esp\u00edrito Santo [Cities: Esp\u00edrito Santo](IBGE 2025) https:\/\/cidades.ibge.gov.br\/brasil\/es\/panorama."},{"key":"e_1_2_12_15_1","unstructured":"L. R.Feitoza L. L. F.Castro M.Resende et al. Mapa das Unidades Naturais do Estado do Esp\u00edrito Santo: informa\u00e7\u00f5es b\u00e1sicas(Incaper 2010) geobases.es.gov.br."},{"key":"e_1_2_12_16_1","unstructured":"Instituto Brasileiro de Geografia e Estat\u00edstica (IBGE) Cidades: Vit\u00f3ria [Cities: Esp\u00edrito Santo](IBGE 2025) https:\/\/cidades.ibge.gov.br\/brasil\/es\/vitoria\/panorama."},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2021.101542"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.066"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-024-10350-x"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163962"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.191"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty191"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr509"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)00308-X"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.03773"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600755453"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_2_12_32_1","unstructured":"A.Rambaut FigTree Version 1.4.4(Institute of Evolutionary Biology University of Edinburgh 2018)."},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12628"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mss075"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adq4326"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2021.04.010"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/v17010057"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofx056"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0837-2020"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26955"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.61921"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2023.105599"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty407"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01183-24"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-024-01116-4"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1393-9"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35622-x"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-0711-8"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed8060325"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/ve\/vead059"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.17843\/rpmesp.2024.414.13898"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3001.230972"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00256-21"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.71078","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.71078","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.71078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:28:08Z","timestamp":1787664488000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.71078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":53,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jmv.71078"],"URL":"https:\/\/doi.org\/10.1002\/jmv.71078","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]},"assertion":[{"value":"2026-01-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71078"}}